Can a polyclonal AB distinquish between two very similar proteins
Alexander P Rostovtsev
rosto001 at maroon.tc.umn.edu
Thu Jun 23 15:51:21 EST 1994
In <2uaoqa$oec at matt.ksu.ksu.edu> jaishre at ksu.ksu.edu (Jaishree Muppala Chittoor) writes:
>Can a polyclonal AB raised against a synthetic peptide (MNLAPLDT) be able
>to distinguish between a protein with this sequence and a protein that
>shows >70% sequence identity with it. This other protein has SNLAPVDT as its
>sequence at the corresponding region.
>Thanks in advance.
>[jaishre at matt.ksu.edu]
Yes, they can. Polyclonal Abs against Tyr-Gly-Gly recognized [met]- and
[leu]enkepalins and lots of other stuff.
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