Can a polyclonal AB distinquish between two very similar proteins

Alexander P Rostovtsev rosto001 at maroon.tc.umn.edu
Thu Jun 23 15:51:21 EST 1994


In <2uaoqa$oec at matt.ksu.ksu.edu> jaishre at ksu.ksu.edu (Jaishree Muppala Chittoor) writes:


>Hi,
>Can a polyclonal AB raised against a synthetic peptide (MNLAPLDT) be able
>to distinguish between a protein with this sequence and a protein that 
>shows >70% sequence identity with it.  This other protein has SNLAPVDT as its 
>sequence at the corresponding region.
>Thanks in advance.
>bye
>Jaishree
>[jaishre at matt.ksu.edu]

Yes, they can. Polyclonal Abs against Tyr-Gly-Gly recognized [met]- and
[leu]enkepalins and lots of other stuff.

AR




More information about the Methods mailing list