sick JM109's

Frances Hannan Zoology flh at
Thu Jun 23 06:48:26 EST 1994

I am having a problem with my JM109's. I can't make them CaCl2 competent 
anymore! I maintain the bugs on minimal plates with thiamine so they 
should still have their F'. The CaCl2 procedure works OK for XL1-blue's 
so that is not the problem either. Has anyone else experienced this? 
Does anyone in Cambridge (UK) have a stock that I can take a streak from?

Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at                               

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