Protocol needed for raising Ab. to overexpressed proteins

liyer at liyer at
Thu Jun 23 11:07:00 EST 1994

Dear Netters,
I plan to raise antibodies to some overexpressed proteins. I need some
protocol/information on how to purify these proteins before injecting it 
into rabbits. Can one use the inclusion bodies for raising antibodies?
Thankyou in advance,
Please reply to 
liyer at
Yours sincerely,
Lakshminarayan Iyer 

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