Can a polyclonal AB distinquish between two very similar proteins

Michael Holland M.Holland at
Thu Jun 23 18:04:03 EST 1994

In message 22 Jun 1994 20:34:02 -0500,
  jaishre at (Jaishree Muppala Chittoor)  writes:

> Hi,
> Can a polyclonal AB raised against a synthetic peptide (MNLAPLDT) be able
> to distinguish between a protein with this sequence and a protein that
> shows >70% sequence identity with it.  This other protein has SNLAPVDT as
> its  sequence at the corresponding region.
> Thanks in advance.
> bye
> Jaishree
> [jaishre at]
This is very difficult to predict. It depends on how many B cell epitopes
are contained within the first peptide. If there are several and one (or
more) are also contained within the second peptide it is likely they will
interact ASSUMING that the second peptide is in a part of the protein that
has high surface probability and is thus available to the immune system. It
is this last point which is most likely to be a problem and thus give you
problems! You can run all the hydropathy plots, surface probability plots
etc but I think our understanding of this is really so superficial that such
endeavours are of more psychological than physiological worth. That only
leaves try it and see!!
Good luck
Michael K Holland PhD
CSIRO, Wildlife & Ecology
PO Box 84, Lyneham ACT 2602, Australia
Fax: 61-6-242-9242  Phone:61-6-242-1793
mholland at

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