Why do cell cultures (sometimes) foam

John E. Fox altabios at bham.ac.uk
Fri Jun 24 07:04:24 EST 1994

In article <YMH4375-230694152329 at mac2wild2.tamu.edu>, YMH4375 at zeus.tamu.edu (Yasha Hartberg) says:
>Several people in our lab have recently been distressed to find that some
>of their liquid cell cultures foam and some do not.  There doesn't seem to
>be any consistant reason we can find for this phenomenon.  With one of our
>cell lines, two identical liquid cultures can be inoculated from the same
>colony and grown for the same length of time under the same conditions and
>one culture will foam and another will not.  As far as we can tell, time of
>growth, speed of shaking, and composition of media do not seem to matter.
>My question then is simply why do cell cultures foam in the first place and
>why can two seemingly identical cultures show two different phenotypes?
>Thanks in advance for any light you can shed on this topic for us.
>Yasha Hartberg
>Texas A&M University

I suspect it is due to extra cellular proteins and peptides.
All our synthetic peptides foam in aqueous solution.

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