DNAse I shotgun MnCl2

[P Watson]

Hi

I've been doing partials with DNAse I and Mn2+ with some success but

noticed that there was a discolouration of the buffer and DNA ppt when

I froze the reaction overnight prior to extraction. Is this some oxidation

effect of the Mn? or maybe my Mn is just old? Is the DNA now stuffed?

Any ideas or anecdotes welcome

Thanks

Phil


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Phil Watson
Department of Medicine
University of Sheffield
Clinical Sciences Centre
Sheffield S5 7AU

md1pw at sunc.sheffield.ac.uk

mbpfw at s-crim1.daresbury.ac.uk