TCA on nucleic acid

dkim at unm.edu dkim at unm.edu
Fri Jun 24 16:07:03 EST 1994


drsmith at shaman.nexagen.com (Drew Smith) says:

"At low pH, the phosphate backbone of the DNA is protonated, causing it to
become uncharged, and thus insoluble in water - this is why it precipitates."

Is this also why DNA goes into the phenol/interphase in the acid guanidine
RNA preparations?  If so, how does RNA avoid this fate?

Daniel kim



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