CAT + Luciferase same buffer

[Jorge L. Sepulveda]

Hi, netters:

Does anyone have experience in doing the protein extraction for the CAT 
and luciferase assays in the same buffer? Apparently, the ideal prep for 
luciferase requires detergents and most detergents inhibit CAT.

Thanks for your help!


Jorge L. Sepulveda, 

Dept. of Pathology,                        Email: Jorges at bcm.tmc.edu
Baylor College of Medicine                 Telephone: 713-798-7330
One Baylor Plaza, Houston, TX 77030        Telefax: 713-798-5838