TCA on nucleic acid

Dongxian Yue dxy at iastate.edu
Sat Jun 25 15:38:33 EST 1994


In article <2ud4mc$co2 at dingo.cc.uq.oz.au> eqfvari at dingo.cc.uq.oz.au (Frank Vari) writes:
>Could anyone please tell me what the effect of 10% aqueous trichloroacetic
>acid (TCA) is on nucleic acids? Are there any good references that someone
>could point me in the direction of? Please Email me or post a response here. 
>
>Many thanks. 

Under 5--10% TCA, RNA/DNA can bind to filter paper by forming weak ester
bonds. So you can wash away the small aminoacid or other ligands. In practice
the nucleic acids longer than 20 bases can bind efficently. It will degrade in
such low pH, normally do it  at 4C.

Yue

  

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