Isolating monocytes from peripherla blood?

[Donald Chun Kit Wong]

We need to isolate monocytes from peripheral blood. Is there any method 
that are good to use? We've read about the density gradients from Gibco 
and Accurate Chemicals. They seem easy to use but only recover about 60% 
of the monocytes. Is there any other methods people routinely use? Thanks.
 
Donald Wong
userDONO at mtsg.ubc.ca
Pathology, UBC