About Compressions with Sequencing

Richard Near rocket1 at bu.edu
Sat Jun 25 23:56:46 EST 1994


You could try PCR sequencing (many kits are available) or even
Maxam Gilbert sequencing (more difficult but not subject to
enzymatic problems-- I recently saw a kit advertized I think in
Biotechniques.

As for gel conditions, I would first try to get the gels to
run at 55 C before I would try things like the formamide gel.
Also, sequence both strands-- what is unreadable in one strand
is often easily readable in the other.

good luck
rick
rocket1 at bu.edy




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