mRNA isolation kits
Rich Maki's lab
rmaki at ljcrf.edu
Sat Jun 25 00:29:12 EST 1994
In article <2u6h85$s7f at nuscc.nus.sg>, med30065 at leonis.nus.sg (TAN MIN-HAN)
> Hi people,
> I'm a student working on the isolation of mRNA from tissue, and I'd
> really appreciate it if anyone could give me an idea of all the
> commercial mRNA isolation kits available. My intention is to test out the
> yield of each of these kits.
> From a quick browse through the advertisements of the past issues of
> Nature, I've found 1) Promega's PolyATract mRNA isolation system 2)
> QIAGEN's OligoTex 3) TRI reageant from Molecular Research Centre
> If anyone knows of any other kits, or uses them for mRNA extraction, I'd
> really appreciate it if you could tell me what they are. Thank you very
> Tan Min-Han Faculty of Medicine
> E-mail: med30065 at leonis.nus.sg National University of Singapore
> minhan at biomed.nus.sg
> --Hold Infinity in the palm of your hand, and Eternity in an hour--
Hi, Tan Min-Han
We use a kit called RNAzol B, manufactured by Bioteck Laboratories Inc
(Telephone number 1 (800) 535-6286; Fax number 1 (713) 643-3143. It is a
really quick protocol, which allows you to extract total RNA from tissues
or culture cells in less than 2 hours, and starting with 10.000.000 cells
we have managed to extract up to 50 micrograms of total RNA.
We hope this information is useful to you.
Annabel and Martin.
More information about the Methods