Transfection efficiency for CAT assays

Winoto lab lab_winoto at maillink.berkeley.edu.
Mon Jun 27 18:21:55 EST 1994


In article <tsichlis-270694120912 at thalis.rm.fccc.edu>
tsichlis at anaximander.rm.fccc.edu () writes:

> I'm doing CAT assays in Jurkat and EL4 (T-lymphocyte) lines.  Transfection
> is via electroporation (its not great but it works--if you have a method
> you love please send it).
> Recently I've started adding expression constructs to check activity on the
> CAT reporters.  The problem is that they are also affecting the
> CMV-betagalactosidase transfection efficiency control.
> I thought about doing dot-blot quantitation of the CAT gene in Hirt
> supernatants from the transfectants.  Is this a good idea?  Has anyone out
> there thought of a better way to control for these assays

Lee,

I sent you a FAX on this, hope you got it.

Paul W. Diaz 



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