Transfection efficiency for CAT assays

[Winoto lab]

In article <tsichlis-270694120912 at thalis.rm.fccc.edu>
tsichlis at anaximander.rm.fccc.edu () writes:

> I'm doing CAT assays in Jurkat and EL4 (T-lymphocyte) lines.  Transfection
> is via electroporation (its not great but it works--if you have a method
> you love please send it).
> Recently I've started adding expression constructs to check activity on the
> CAT reporters.  The problem is that they are also affecting the
> CMV-betagalactosidase transfection efficiency control.
> I thought about doing dot-blot quantitation of the CAT gene in Hirt
> supernatants from the transfectants.  Is this a good idea?  Has anyone out
> there thought of a better way to control for these assays

Lee,

I sent you a FAX on this, hope you got it.

Paul W. Diaz