Transfection efficiency for CAT assays
lab_winoto at maillink.berkeley.edu.
Mon Jun 27 18:21:55 EST 1994
In article <tsichlis-270694120912 at thalis.rm.fccc.edu>
tsichlis at anaximander.rm.fccc.edu () writes:
> I'm doing CAT assays in Jurkat and EL4 (T-lymphocyte) lines. Transfection
> is via electroporation (its not great but it works--if you have a method
> you love please send it).
> Recently I've started adding expression constructs to check activity on the
> CAT reporters. The problem is that they are also affecting the
> CMV-betagalactosidase transfection efficiency control.
> I thought about doing dot-blot quantitation of the CAT gene in Hirt
> supernatants from the transfectants. Is this a good idea? Has anyone out
> there thought of a better way to control for these assays
I sent you a FAX on this, hope you got it.
Paul W. Diaz
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