Can a polyclonal AB distinquish between two very similar proteins

Mike Preston preston at warren.med.harvard.edu
Mon Jun 27 17:01:56 EST 1994


In article <2uaoqa$oec at matt.ksu.ksu.edu>, jaishre at ksu.ksu.edu (Jaishree
Muppala Chittoor) wrote:

> 
> Hi,
> Can a polyclonal AB raised against a synthetic peptide (MNLAPLDT) be able
> to distinguish between a protein with this sequence and a protein that 
> shows >70% sequence identity with it.  This other protein has SNLAPVDT as its 
> sequence at the corresponding region.
> Thanks in advance.
> bye
> Jaishree
> [jaishre at matt.ksu.edu]

Jaishree,

To answer this question, why not do a competitive inhibition ELISA.  Take
your polyclonal sera and try to inhibit the binding of it to your "MNLAPDT"
protein (which has been coated onto the ELISA plate) by adding dilutions of
the "other" protein to your sera before adding it to the ELISA plate.

Good luck

Mike Preston
preston at warren.med.harvard.edu
617-432-0152



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