Can a polyclonal AB distinquish between two very similar proteins

Tom Chappell dmcbtch at ucl.ac.uk
Tue Jun 28 09:08:01 EST 1994


Jaishree,

It depends a bit on exactly what you are trying to do, but if you blot
a partial proteolysis gel, you should have no problem distinquishing
between 2 proteins with 70% sequence identity using your peptide
antibody. I've used this approach with HSP70's and can easily see
differences.


Tom Chappell
MRC Lab for Mol. Cell Biol. 



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