pCAT Basic cut by Hind III

[Winoto lab]

In article <CrKC86.3FD at acsu.buffalo.edu>
vp23qw83 at ubvms.cc.buffalo.edu (Xin Guo) writes:

> cloning. I have no idea what the 8.8kb band is. Is it possible
> that the band is ligated two linear 4.4kb DNA or something else?
> I isolated the pCAT from E. coli using phenol/choloroform method.
  

I agree with Chris, partial digestions are often caused by the
conditions chris mentioned.

Pendragon