pCAT Basic cut by Hind III

Winoto lab lab_winoto at maillink.berkeley.edu.
Tue Jun 28 19:13:06 EST 1994

In article <CrKC86.3FD at acsu.buffalo.edu>
vp23qw83 at ubvms.cc.buffalo.edu (Xin Guo) writes:

> cloning. I have no idea what the 8.8kb band is. Is it possible
> that the band is ligated two linear 4.4kb DNA or something else?
> I isolated the pCAT from E. coli using phenol/choloroform method.

I agree with Chris, partial digestions are often caused by the
conditions chris mentioned.


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