West blotting

[S. Xu]

Hi, Folks,

I am interested in protein tyrosine phosphorylation in megakaryocyte
differentiation.  Currently I have a problem doing Western blotting.  I
used "Wet Electrophoric Transfer" method, but I found that the bands
with low molecular weight were not evenly transferred.  BTW, I was told
that it was very tricky to study tyrosine phosphorylation by using
Western blotting, which I just touched.  Any help will be appreciated.

Thanks in advance.

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Ying Hong       Dept of Medicine
                King's College School of Medicine
                London SE5 9PJ
                Email: RCHA213 at MAPL.KCL.CC.AC.UK
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