Help with large SDS-PAGE gels

[Gus Trautweiler]

Receiving very poor resolution for mw proteins less than 25000 for gels 22cm x
18cm.  Smaller gels (12cm x 8cm) work.  Stacking gel
conc.= 5% and tris pH6.8, running gel conc. have been run at 12%, 15%, 20% and
tris pH8.8. Voltage was 100 stepped to 200 with milliamps at 30 maximum.  Total
time of run approx. 5hr.  Thank you for your help, Timothy J. Byrne

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