Help with large SDS-PAGE gels

Gus Trautweiler AAT at UCSVAX.UCS.UMASS.EDU
Wed Jun 29 11:44:35 EST 1994


Receiving very poor resolution for mw proteins less than 25000 for gels 22cm x
18cm.  Smaller gels (12cm x 8cm) work.  Stacking gel
conc.= 5% and tris pH6.8, running gel conc. have been run at 12%, 15%, 20% and
tris pH8.8. Voltage was 100 stepped to 200 with milliamps at 30 maximum.  Total
time of run approx. 5hr.  Thank you for your help, Timothy J. Byrne

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Plant & Soil Science Dept: Biotechnology|  |  | | # | |  |  |  #  |  |  |
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AAT at BIO.UMASS.EDU   *##*      *##*         *##*       *##*           *##*



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