Chomczynski protocol

BIOMETRA at BIOTECHNET.COM BIOMETRA at BIOTECHNET.COM
Wed Jun 29 06:14:29 EST 1994


Hi Netters,
	Has anyone used the Chomczynski protocol that appeared in
Biotechniques(1993) vol 15. no. 3 page 532.  This procedure is supposed
to simultaneously isolate RNA, DNA and protein.  I have seen an add in
Science from a company called Molecular Research Center that sells
a kit for this called Tri Reagent.  Has anyone used the kit?  Does
this procedure really work?  How are the yields of each? Purity?  Can
the DNA and RNA be further manipulated (PCR, Restriction Digests, Ligation)


Tony



More information about the Methods mailing list