Isolation of Total RNA from just about anything

Winoto lab lab_winoto at maillink.berkeley.edu.
Wed Jun 29 14:09:34 EST 1994


In article <2us1uv$quh at tamsun.tamu.edu>
acc4636 at tamsun.tamu.edu (Anthony C.) writes:

>  At this point I dont care if the prep is partially
> digested with RNAses.. I just want the smear!  If you have any suggestions
> let me know! 

If all you want to do is observe the quantity/quality of RNA just
resuspend the RNA is formamide dye and run it out on a regular 1%
agarose gel, run the gel at 100-150 volts for 20 min.  The RNA doesn't
degrade that fast.  I do this all the time and I can see the RNA just
fine.

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