purify PCR products using Wizard Prep (Promega)

Winoto lab lab_winoto at maillink.berkeley.edu.
Wed Jun 29 14:16:07 EST 1994


In article <2urvht$c1b at triton.unm.edu>
dkim at unm.edu writes:

> Finally, people often get acceptable yields using straight physical methods
> for DNA from gel purification, such as freeze and squeeze, etc. You might want
> to check out a very quick and dirty method in BioTechniques (sometime this
> year) in which the gel slice is simply pushed through a 0.45 micron syringe
> filter.
> 
> Daniel Kim

Dan,

I've tried the squeeze method you mentioned and was never able to get
it to work that well.  Have you tried it?  What kind of results did you
get?

Pendragon 



More information about the Methods mailing list