llintott at ACS.UCALGARY.CA
Thu Jun 30 10:53:38 EST 1994
> We are having terrible problems with the BIO101 "Geneclean" kit.
> We have tested different plasmids, rest. enzymes, researcher'hands
> and so on but we never obtain purified bands which can ligate efficiently.
> Apparently the problems started a couple of months ago (march-April 94)
> and we wonder if it has something to do with new batches of the kit shipped to Europe (Italy).
> We usually purify our DNA bands from Ultra Pure agarose (BRL) gel run in
> TBE buffer.
> Any suggestion is gratly welcome !!!!
> Daniela Marazziti
> Insti. of Cell Biology, CNR, Viale Marx 43, I-00137 Rome
> fax: +39-6-8273287
> e-mail: daniela at biocell.irmkant.rm.cnr.it
You can not use TBE buffer to run gels which you want to purify
bands from. Try a buffer such as TAE.
50 X TAE
2M Tris base
0.05 M EDTA (pH 8.0)
57.1 ml Glacial Acetic Acid (per L)
Bio. Sci. U of Calgary
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