geneclean problems

[Lauri Lintott]

> 
> We are having terrible problems with the BIO101 "Geneclean" kit.
> We have tested different  plasmids, rest. enzymes, researcher'hands
> and so on but we never obtain purified bands which can ligate efficiently.
> Apparently the problems started a couple of months ago (march-April 94)
> and we wonder if it has something to do with new batches of the kit shipped to Europe (Italy).
> We usually purify our DNA bands from Ultra Pure agarose (BRL) gel  run in 
> TBE buffer.
> Any suggestion is gratly welcome !!!!
> 
> Daniela Marazziti
> Insti. of Cell Biology, CNR, Viale Marx 43, I-00137 Rome
> fax: +39-6-8273287
> e-mail: daniela at biocell.irmkant.rm.cnr.it
> 
> 
You can not use TBE buffer to run gels which you want to purify
bands from.  Try a buffer such as TAE.
50 X TAE
2M Tris base
0.05 M EDTA (pH 8.0)
57.1 ml Glacial Acetic Acid (per L)

Lauri Lintott
Bio. Sci. U of Calgary