T7 RNA polymerase inhibitor

[Peter Gegenheimer]

In <2E1098C2 at smtpgate.lifesci.ucla.edu>, HarryY at MICROBIO.LIFESCI.UCLA.EDU ("Yim, Harry               M'bio") writes:
>Does anyone know of an EFFECTIVE inhibitor of T7 RNA polymerase?  
>Specifically I am looking for something that inhibits initiation but 
>not elongation.  I am studying RNA folding and so an additional requirement 
>would be that it not affect RNA:RNA interactions.  I have searched in the 
>literature and there doesn't seem to be anything worthwhile.  Thanks.
>
>Harry Yim
>UCLA
>

Yes, T7 lysozyme is an effective inhibitor of T7 RNAP. It certainly inhibits
initiation, and I expect -- but don't know -- that it cannot attach to 
elongating RNAP. Since it is not toxic to E. coli, it probably has no gross
effect on RNA:RNA interactions. It is available from Bill Studier or from 
Novagen as clone pLysE, in E. coli BL21(DE3)/pLysE.

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