Geneclean problems

James S. Sutcliffe jamess at mbcr.bcm.tmc.edu
Thu Jun 30 15:20:37 EST 1994


In article <2uv43s$j5p at news.mic.ucla.edu>, Robert Lloyd <rlloyd at inherit2.dna.ucla.edu> writes:
|> In article <2uukkl$bom at mserv1.dl.ac.uk> Dr. D. Marazziti,
|> dmarazzi at crc.ac.uk writes:
|> 
|> ....
|> 
|> >We usually purify our DNA bands from Ultra Pure agarose (BRL) gel  run
|> in 
|> >TBE buffer.
|> 
|> The instructions that came with the geneclean kit I have suggest
|> (strongly)  not to use TBE.  I have good success with TAE.  Hope this
|> helps.


This is true, but doesn't the Geneclean II kit provide additional reagent(s)
to allow you to use TBE. So, the question is were they using the Geneclean I
or Geneclean II kits. I agree with you, however, I always use TAE and have
good success.



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