Methods June 1994 Archives by author

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Starting: Wed Jun 1 00:06:10 EST 1994
Ending: Thu Jun 30 22:52:31 EST 1994
Messages: 1038

• Regs for mailing DNA   "Mary Colter ", BIO
• Deletion problems   "jeffrey wigle ", grad stud
• Appropriate distance b2t rib binding site & ATG?   Stefan Gruenert (WCI)
• Help needed to re-cycle QIAGEN <plasmid> tips  
• Buffering Phenol  
• quantifying of nucl. acids  
• RNAse stability  
• test  
• insoluble DNA pellet after Qiagen Tip20  
• Regs for mailing DNA  
• Vent PCR Woes   191502931
• PFGE of Clostridium and Bacillus   Enrique Jose Labadan Frio - SCBC - 3421101
• Cyanobacterial Promoters   Enrique Jose Labadan Frio - SCBC - 3421101
• Water used in PCR   4700gbera at UMBSKY.CC.UMB.EDU
• RNAse stability   4700gbera at UMBSKY.CC.UMB.EDU
• Black DNA from earthworms   4700gbera at UMBSKY.CC.UMB.EDU
• PCR for Random Mutagenesis - Help!   5978jsnyd at UMBSKY.CC.UMB.EDU
• subscribe   5978jsnyd at UMBSKY.CC.UMB.EDU
• human c-fos probe information   ALLEN, BEVERLY S
• gels sticking to glassplate   APARKER at MAINE.MAINE.EDU
• luciferase in vivo in mammals?   David Adelson
• summary of luciferase in mammalian cell replies to date.   David Adelson
• Inducible eukaryotic expression vector ?   Kari Airenne
• nitrosoguanidine   Jay Allen
• Buffering Phenol   Jay Allen
• reusing agarose gels   Paul St. Amand
• Moravek Biochemicals address   Dean R. Appling
• Which electroporator to buy?   Tracy Aquilla
• Which electroporator to buy?   Tracy Aquilla
• how to separate the nicked from of a plasmid from the closed form?   Tracy Aquilla
• dissection and RNA extraction   Tracy Aquilla
• PCR primers for ribosomal RNA   Tracy Aquilla
• Altered Sites II- Any good?   Tracy Aquilla
• house keeping standard   Tracy Aquilla
• Why do cell cultures (sometimes) foam   Tracy Aquilla
• PCR ghost bands   Tracy Aquilla
• gels sticking to glassplate   Tracy Aquilla
• purify PCR products using Wizard Prep (Promega)   Tracy Aquilla
• reusing agarose gels   Armadin
• Help: no transformants using BaculoGold   Curt Ashendel
• Water purification systems-Which is best?   Curt Ashendel
• The end of inclusion bodies?   Graham Atherton
• The end of inclusion bodies?   Graham Atherton
• ethidium bromide disposal: a caveat   B.L.Cohen
• ethidium bromide disposal   TOM BICKLE
• Biometra is now online   BIOMETRA at BIOTECHNET.COM
• Chomczynski protocol   BIOMETRA at BIOTECHNET.COM
• how to find the vendor   DAVID F. BISHOP
• Expression from Bluescript   BSS032 at BANGOR.AC.UK
• LUCIFERASE woes   Charolyn Babichuk
• Buffering Phenol   Joseph C. Bagshaw
• QIAGEN resin   Joseph C. Bagshaw
• QIAGEN resin   Joseph C. Bagshaw
• RNA isolation request   Joseph C. Bagshaw
• Black DNA from earthworms   Joseph C. Bagshaw
• TCA on nucleic acid   Joseph C. Bagshaw
• phone books   Allen Bartman
• Help needed to re-cycle QIAGEN <plasmid> tips   Dave Bates
• Kanamycin stability at 80C   Dave Bates
• Detecting b-gal in cultured cells ?   Dave Bates
• Help with large SDS-PAGE gels   Brian J. Baumgartner
• RNase inhibitor   Hinayana Bawagan
• Transfection of Sf9/Sf21 Cells   Hinayana Bawagan
• in vitro transcription: low incorporation of label   Hinayana Bawagan
• ROCHE e-mail address   Hinayana Bawagan
• MYCOPLASMA testing with PCR - advice sought...   Clifford J Beall
• Eastern blotting!   Ed Beaty
• Benchtop oligos: fast and cheap?   Ed Beaty
• Benchtop oligos: fast and cheap?   Ed Beaty
• Surf Blot Experience   Ed Beaty
• books on PCR   Ed Beaty
• multiple antigenic peptides - MALDI   Ron Beavis
• ethidium bromide disposal   J. Bechtold
• cross linkining   Richa Behari
• Which Thermal Cycler   Christian Beltinger
• Request: CCD camera sources   Ethan Benatan
• luciferase in vivo in mammals?   Michael Benedik
• PCR mutagenesis   Michael Benedik
• Broad host range cosmid, with Km resistance   Michael Benedik
• Request: CCD camera sources   Gregory Benjamin
• Auto-DNAprep machines   Jack S. Benner
• Yakult Honsha address wanted   Ron Berry
• retrovirus hazards   Dr. S. Bhattacharya
• PCR on whole yeast cells   Robert Solomon Bioc
• purify PCR products using Wizard Prep (Promega)   Robert Solomon Bioc
• purify PCR products using Wizard Prep (Promega)   Robert Solomon Bioc
• Microplate fluorometer favourites?   Inst. f. Biochemie
• Address needed: Avanti Polar Lipids   Inst. f. Biochemie
• detection of apoptosis, new methods   Inst. f. Biochemie
• Detection of SV40largeT and Amonioglycosid-phosphotransferase   Inst. f. Biochemie
• What is the difference between minisatellite and microsatellite?   Huang Bixing
• dilution for western   Shaun D. Black
• detergent removal help!   Shaun D. Black
• Fusion proteins for eukaryotic expression?   John E. Blume
• Which thermal cycler to buy??   Peter T. Boag
• DNA folding   Alex Borovkov
• Coli Genetic Stock Center - on-line??   Dave Boyd
• Protein seq companies in Canada?   Dave Boyd
• purify PCR products using Wizard Prep (Promega)   Dan Bradley
• how to separate the nicked from of a plasmid from the closed fo   BrianC4208
• Slanted Bands on Agarose Gels   Thomas A. Brown
• FIAU   Lisa J Brunet
• Karyotyping   Lisa J Brunet
• ?Mouse breeding database   Lisa J Brunet
• Vent PCR Woes   John Brunstein
• bizzare digestion problem   John Brunstein
• PAPAIN   John Brunstein
• bizzare digestion problem-solved?   John Brunstein
• Long PCR smear   Anne Buchanan
• magnetic latex ?   Sebastian Bunka
• magnetic latex/polysciences reply   Sebastian Bunka
• Solvent for D-Biotin?   Sebastian W. Bunka
• DNA extraction from P. fluorescens   Sebastian W. Bunka
• Drying gels w/o vacuum?   Sebastian W. Bunka
• using ABTS with HRP-conj for immunoscreening of lambda library   Sebastian W. Bunka
• Determining Genome Sizes   Timothy Bushnell
• Looking for MJ Research address   Tim Buss
• pET-3d vector sequence   Stefanie Butland
• Isolation of Total RNA from just about anything   Anthony C.
• FILM COLOR VIA DAVID KATZ   CAWHC at CUNYVM.CUNY.EDU
• lambda gt6   CLONTECH at BIOTECHNET.COM
• hygromycin phosphotransferase   Glenn Cantor
• color selection with omega subunit?   Darrick Carter
• About Compressions with Sequencing   Darrick Carter
• Zoo blots   Elisabeth Casademunt
• DNA extraction from P. fluorescens   Ron Caspi
• Broad host range cosmid, with Km resistance   Ron Caspi
• Library search.   Heather Catherine
• Thermal Cyclers   KUN Celbiologie
• FLAG Shift 12/12C   George W Chacko
• Inducible eukaryotic expression vectors   Daniel Chagnovich
• Buffering Phenol   Dr. C.J. Chan
• Cheap and functional electroporator   Dr. C.J. Chan
• patent for SDA and LCR   Alex Chang
• Eastern blotting!   Tom Chappell
• Can a polyclonal AB distinquish between two very similar proteins   Tom Chappell
• Which protein seq. lab service??   Peter Charlton
• (none)   Shao-Yu Chen
• boyden chamber   Shu-Chih Chen
• how to find the vendor   Shu-Chih Chen
• Western Conference of Immunology   Vincent Y. Chen
• Radio-sequencing   Andrew Cherniack
• Can a polyclonal AB distinquish between two very similar proteins   Jaishree Muppala Chittoor
• Taq Extender   Maninder Chopra
• Gopher for the DSM   Duncan Clark
• Dyes and DNA PAGE   Duncan Clark
• Gopher for the DSM   Duncan Clark
• Search Genbank via gopher   Duncan Clark
• Sequence alignment   Duncan Clark
• Pfu-spiked cycle sequencing   Duncan Clark
• Coli Genetic Stock Center - on-line??   Duncan Clark
• Pfu-spiked cycle sequencing   Duncan Clark
• Tth 111 II   Duncan Clark
• PCR machine patents   Duncan Clark
• Using HP scanjet as densitometer   Duncan Clark
• Ligation of PvuI site   Duncan Clark
• Linker interferes w Transformation?   Duncan Clark
• Sequence of pTTQ18   Duncan Clark
• Autoclaving DNA and contamination   Duncan Clark
• Autoclaving DNA and contamination   Duncan Clark
• Sequence of pTTQ18   Duncan Clark
• Qiagen oligotex mRNA kit   Sonya Clark
• RNA internal standard   D L Clarke
• PCR with ubiquitous primers   Michael Coady
• Black DNA from earthworms   Andrew Cockburn
• PCR final hold temp. article???   Andrew Cockburn
• QIAGEN resin   Nicola Collie
• RNAse protection kits   Steven Coon
• Anchor PCR question   Bob Corell
• I need a complete GAL4 clone   Bob Corell
• mRNA isolation from total RNA Kits (NEW)   Matt S. Crane
• potato acid phosphatase   Kirsten Crossgrove
• potato acid phosphatase   Kirsten Crossgrove
• Cross-linking   SCOTT D.
• PCR Trouble   Scott Wildenberg DGM-IHG
• Glacial Acetic Acid   Dr R. Dalgleish
• Removing Bind Silane from plates   Dr R. Dalgleish
• Removing Bind Silane from plates   Dr R. Dalgleish
• pET, lysE, lysS, bacterial expression questions   Bipin K Dalmia
• GST fusion proteins and dnaK   Bipin K Dalmia
• RNAse protection kits   Damak, Sami
• RNase inhibitor   Damak, Sami
• To Pichia or not to Pincha??   Damak, Sami
• dissection and RNA extraction   Damak, Sami
• pCAT Basic cut by Hind III   Damak, Sami
• RNA extraction from fixed tissue   Damak, Sami
• Purification of small DNA   Richard Dana
• Plant DNA   Richard Dana
• cDNA library methods -plants   Richard Dana
• Deletion problems   Hong Dang
• Vent PCR Woes   Hong Dang
• Blunting PCR products with Pfu.   Hong Dang
• DNA extraction from P. fluorescens   Darren.Martin
• 3 & 5' Overhangs...   David L. Haviland, Ph.D.
• Looking for OSP - a primer application   David L. Haviland, Ph.D.
• PCR of episomal plasmids   David L. Haviland, Ph.D.
• Water used in PCR   David L. Haviland, Ph.D.
• Buffering Phenol   David L. Haviland, Ph.D.
• Gel dryer / pump corrosion -   David L. Haviland, Ph.D.
• RT-PCR problem.....HELP!   Simon Dawson
• Post doc position   James D DeVault
• Long PCR stuck in the wells :-( (fwd)   Dan Diaz
• Thermal Cyclers   Marcelo C. Dornelas
• ethidium bromide disposal   Julian Dow
• polyacrylamide-agarose gel   Virginia Dress
• DNA PAGE EtBr query.   Virginia Dress
• T7 promoters and expression   Virginia Dress
• PCR on whole yeast cells   Dr. I. Dunham
• HIS TAG and antibody affinity purification   STRUCTURAL NEUROBIOLOGY XTAL LAB- MADE FROM 100% RECYCLED ELECTRONS
• insoluble DNA pellet after Qiagen Tip20   Jan Eggermont
• Transfection with Lambda   Bruce Elder
• mtDNA Prep   Bruce Elder
• Quick and cheap +/- PCR screen?   Elmer M. Price, Ph.D.
• Transfection of Sf9/Sf21 Cells   Elmer M. Price, Ph.D.
• bizzare digestion problem   Elmer M. Price, Ph.D.
• Linker interferes w Transformation?   Elmer M. Price, Ph.D.
• PCR mutagenesis   Elmer M. Price, Ph.D.
• SDS-PAGE-resistant protein complexes   Elmer M. Price, Ph.D.
• Water purification systems-Which is best?   Elmer M. Price, Ph.D.
• Prep of M13 RF DNA   Elmer M. Price, Ph.D.
• gels sticking to glassplate   Elmer M. Price, Ph.D.
• RecPhage/ScFv Ab methods?   Peter Emanuel
• Microbiology Newsgroup Vote   the End
• Okay, Okaaaay!. Here's the recipe for Non ppt 10x TBE   the End
• Buffering Phenol: pH directly   the End
• Alkaline lysis DNA prep   the End
• PCR machine patents   the End
• reusing agarose gels   the End
• Vent PCR Woes   the End
• Autoclaving DNA and contamination   the End
• Ultra-Micro UV-Vis spec <$3000 ?   the End
• Cloning a PCR product   the End
• gels sticking to glassplate   the End
• Hygromycin   Carol S. Enderlin
• Request: CCD camera sources   Sheldon L. Epstein
• Doubletake mutagenesis kit (Stratagene)   Sigrid Erdal
• cross linking   Tom Esch
• RIPPED FROM TODAY'S HEADLINES: 2 month PCR?   Peter S. Evans
• Optiseals and Needles   FJVAND00 at ukcc.uky.edu
• help lysing E. coli to purify inclusion bodies   FJVAND00 at ukcc.uky.edu
• DNA folding   FORSDYKE at QUCDN.QueensU.CA
• Karyotyping   EDWARD M. FOX
• Automated sequencing of PCR products   Sergi Ferrer
• MitC and EtBr disposal   Sergi Ferrer
• thrombin cleavage site   Fiberman
• Quantitating Radioactive label in DNA and RNA   Nicola Fidler
• Overcoming Sequencing Secondary Structure   Seth Findley
• BRENT Lab pJG4-5 (2 hybrid act. domain vector)BRAIN cDNA Library   Seth Findley
• compressions during sequencing   Seth Findley
• Re-Lifting from Lambda plates?   Seth Findley
• Pharmacia Recombinant Phage Antibody kit?   H. David Fischer
• Newsgroup bionet.molbio.methds-reagnts, Articles 15429-15458-antibodies to Rabs   Parke K. Flick
• Agarose gel electrophoresis on PCR product   Brian Foley
• human c-fos probe information   Brian Foley
• Long RT-PCR   Brian Foley
• PCR of episomal plasmids   Brian Foley
• Long RT-PCR   Brian Foley
• HELP! Odd Gel Pattern!!   Brian Foley
• Intron conservation   Brian Foley
• Long RT-PCR   Brian Foley
• how to separate the nicked from of a plasmid from the closed form?   Brian Foley
• hygromycin phosphotransferase   Brian Foley
• INTRONs CONSERVED!!!   Brian Foley
• Shareware cloning program that reads genbank feature tables?   Brian Foley
• Hyg, Neo, and $$   Brian Foley
• Hyg, Neo, and $$   Brian Foley
• Problems with News archives?   Brian Foley
• Hygromycin resistance as a eukaryotic marker.   Brian Foley
• Autoclaving DNA and contamination   Brian Foley
• PCR,DNA storage for.   Brian Foley
• Autoclaving DNA and contamination   Brian Foley
• Method for Amplified Fragment Length Polymorphism Analysis   Chris Ford
• HELP: Is there any commercial kits for chromosomal DNA isolation from G+ bacteria?   Joe Formanek
• Coli Genetic Stock Center - on-line??   Patricia Foster
• Heat Shock Proteins -- NOT!   Patricia Foster
• ethidium bromide disposal   Patricia Foster
• nitrosoguanidine   Patricia Foster
• ethidium bromide disposal   Patricia L. Foster
• Millipore "EXPIDITE" vs APPLIED BIOSYSTEMS 392   John E. Fox
• Which protein seq. lab service??   John E. Fox
• Weird kinased oligo pattern...   John E. Fox
• multiple antigenic peptides - MALDI   John E. Fox
• Which protein seq. lab service??   John E. Fox
• Glacial Acetic Acid   John E. Fox
• PCR and BrdU   John E. Fox
• Amino acid bonding to glass beads   John E. Fox
• Protein seq companies in Canada?   John E. Fox
• Why do cell cultures (sometimes) foam   John E. Fox
• Ultra-Micro UV-Vis spec <$3000 ?   John E. Fox
• Mammalian cells for high efficiency transfection   Nantel Francois
• Slanted Bands on Agarose Gels   Nantel Francois
• Need help with Institutional Computing Facilities(3X:methods,women,virol)   Marion Freistadt
• Need help with Institutional Computing Facilities(3X:methods,women,virol)   Marion Freistadt
• Isolation of monocytes   Marion Freistadt
• Isolation of monocytes   Marion Freistadt
• Moravek Biochemicals address   Anthony V. Furano
• Buying HeLa Cells $$   Anthony V. Furano
• 2-D sample prep   GAED6043 at VAX1.AGRICULTURE.QUEENS-BELFAST.AC.UK
• Buying HeLa Cells $$   GARCIA02 at UTSW.SWMED.EDU
• AACC Conference info? (New Orleans)   GAWagner
• Isolation of Total RNA from just about anything   DAVID GLOVER
• Geneclean problems   Mr. Steven Swift; BIOL; GRAD
• Seeking photos of Strep. pneumo.   Prasit Palitpolkanpim - GRSO
• Bizzare digestion pattern   GWDGV1::KCHOWDH
• Cellular targets for Large T antigen   GWDGV1::KCHOWDH
• DNA-contaminated PCR   ZOLTAN GYORGYPAL
• Women or Minority owened oligo maker   Jim Gale
• Quick and cheap +/- PCR screen?   Warren Gallin
• Hyb conditions   Warren Gallin
• RT-PCR problem.....HELP!   Warren Gallin
• detergent removal help!   Warren Gallin
• Ampholine manufacturers for IEF   Sally Garrison
• Okay, Okaaaay!. Here's the recipe for Non ppt 10x TBE   Peter Gegenheimer
• pET, lysE, lysS, bacterial expression questions   Peter Gegenheimer
• formaldehyde in RNA gel   Peter Gegenheimer
• help lysing E. coli to purify inclusion bodies   Peter Gegenheimer
• T7 RNA polymerase inhibitor   Peter Gegenheimer
• Seeking photos of Strep. pneumo.   Alan Gerstein
• SDS-PAGE-resistant protein complexes   Allan Gibson
• Search Genbank via gopher   Don Gilbert
• Problems with News archives? IUBio disk failures again   Don Gilbert
• IUBio archive of bionet news is back   Don Gilbert
• southern background problems   Bert Gold
• Very large Plasmid Preps   Roger Graham
• How do you manage your working sample DNA in RAPD?   Margaret Green
• test   Brian Grimwade
• gel imaging   Bob Gross
• purify PCR products using Wizard Prep (Promega)   Xin Guo
• pCAT Basic cut by Hind III   Xin Guo
• Taq Extender   Sunita Gupta
• compressions during sequencing   HARDIES at THORIN.UTHSCSA.EDU
• PCR ghost bands   HARDIES at THORIN.UTHSCSA.EDU
• peptide synthesizers   HRMBDKC at HKUCC.HKU.HK
• HepG2 cells and AFP prod'n   Allison Haggarty
• 24-well petridish, again.   Allison Haggarty
• non-adherent 24-well plates   Allison Haggarty
• Microsatellite oligos   Larry Hale
• wanted DNA from Dunaliella   A.J.W. Hall
• Tth protocol   Dave Hall
• mRNA purification   Peggy Halleck
• polyacrylamide-agarose gel   Andre Hamel
• polyacrylamide-agarose gel   Andre Hamel
• PCR Trouble   Andre Hamel
• Taq Extender   Andre Hamel
• SDS PAGE Taq extender?   Andre Hamel
• Internal ribosomal binding sites, source for   Terry Hanzlik
• ROCHE e-mail address   Rob Harper
• Linker interferes w Transformation?   Yasha Hartberg
• Why do cell cultures (sometimes) foam   Yasha Hartberg
• Where to get protooncogenes ?   A.J. Hartl
• PAPAIN   Dinesh Haryadi
• 3 & 5' Overhangs...   Andrew Healey
• insoluble DNA pellet after Qiagen Tip20   Andrew Healey
• Eastern blotting!   Andrew Healey
• Nusieve Agarose who?*******   Andrew Healey
• Bizzare digestion pattern   Eric J Hebert
• gels sticking to glassplate   Eric J Hebert
• QIAGEN resin   Amos H Heckendorf
• Membrane stripping with the DIG system   Caren Helbing
• Women or Minority owened oligo maker   Sam Henderson
• syringe method for DNA recovery   Paul N Hengen
• Auto-DNAprep machines   Paul N Hengen
• Long RT-PCR   Paul N Hengen
• Which protein seq. lab service??   Paul N Hengen
• Nile red.   Paul N Hengen
• QIAGEN resin   Paul N Hengen
• ethidium bromide disposal   Paul N Hengen
• ethidium bromide disposal   Paul N Hengen
• ethidium bromide disposal   Paul N Hengen
• RNAse stability   Henry
• SDS-PAGE-resistant protein complexes   Bernard Heymann
• "universal" positive control   Walter Hill
• PCR on whole yeast cells   Anthony Hilton
• Quantitating Radioactive label in DNA and RNA   Heidi Lanora Hinkel
• Vent PCR Woes   Tony Hodge
• Gel dryer / pump corrosion - New Pumps from Edwards   Tony Hodge
• Amino acid bonding to glass beads   James A. Holcombe
• Unsubscribe   Michael Holland
• Surf blots   Michael Holland
• To Pichia or not to Pincha??   Michael Holland
• FACE technology   Michael Holland
• Optimal E Coli strains for MBP expression   Michael Holland
• Can a polyclonal AB distinquish between two very similar proteins   Michael Holland
• purify PCR products using Wizard Prep (Promega)   Annette C. Hollmann
• Available mABs anti IgG-FcR II/III of the Rat ??   Gene Holowachuk
• Total phosphate assay?   Louis Hom
• Drying gels w/o vacuum?   Louis Hom
• ethidium bromide disposal   Robert Horton
• ethidium bromide disposal   Robert Horton
• "Standard" housekeeping gene   Robert Horton
• "Standard" housekeeping gene   Robert Horton
• Nile red.   Tony Houthaeve
• ATCC on line   M. L. Huang
• help on direct sequencing of PCR products   M. L. Huang
• Vent PCR Woes   M. L. Huang
• Eastern blotting!   Pierre Hubert
• Help: EGF-receptor purification   Pierre Hubert
• Inducible eukaryotic expression vector ?   David Huen
• detection of apoptosis, new methods   David Huen
• reusing agarose gels   David Huen
• hygromycin phosphotransferase   David Huen
• Hyg, Neo, and $$   David Huen
• Hyg, Neo, and $$   David Huen
• Petri 6well/24well?   David Huen
• Gel dryer / pump corrosion - New Pumps from Edwards   David Huen
• LUCIFERASE woes   David Huen
• Pfu-spiked cycle sequencing   Olav Hungnes
• Pfu-spiked cycle sequencing   Olav Hungnes
• Vent PCR Woes   Olav Hungnes
• HIS TAG and antibody affinity purification   Marko Hyvonen
• how to couple/crosslink nucleic acids?   Horst Ibelgaufts
• Coupling alkaline phosphatase to DNA protokol?   Horst Ibelgaufts
• Eosine/methylene blue agar: origin of metalic colour?   Horst Ibelgaufts
• Sequence of pTTQ18   Ramaswamy K Iyer
• RT-PCR Control Primers?   J.Carnwath
• PCR Trouble -Reply   JENKINS at PCIMSG.PCI.UPMC.EDU
• Oligos labeling   JOEDAG
• Need replacement for Wizards   JOEDAG
• Radiometric detector   JOHNSON, MICHAEL
• Isolation of monocytes   JOHNSON, MICHAEL
• Radiometric detectors   JOHNSON, MICHAEL
• Selection of radiometric detector   JOHNSON, MICHAEL
• using a HP IIp scanner/densitometer   Jack M. Bernstein, M.D.
• nitrosoguanidine   Bill Jack
• Prok. vector x studying 5'UTR (HELP)   Bill Jack
• pET, lysE, lysS, bacterial expression questions   Don Jackson
• Using HP scanjet as densitometer   Don Jackson
• PCR ghost bands   Pierre Jelenc
• PCR ghost bands   Pierre Jelenc
• [Q] Phospho-peptide standards   Ole N Jensen
• FIAU   Joanne_Ownbey at CBRC.MGH.HARVARD.EDU
• Km for B Galactosidase   JoeC503036
• Sequencing Glass Plates   JoeC503036
• Anti Calmodulin   Bo Johansen
• Sequence Analysis Software   Lois C. Johnson
• help lambda-gt11 PCR   Lois C. Johnson
• vacuum or aspirator water pumps purchase   Lois C. Johnson
• How to desiliconise glass plates?   daj (David Johnston)
• "universal" positive control   daj (David Johnston)
• Mol. Dyn. new DNA seq. robot   daj (David Johnston)
• PAPAIN   daj (David Johnston)
• mtDNA Prep   daj (David Johnston)
• About Compressions with Sequencing   daj (David Johnston)
• High MWt Markers   Mandy Johnstone
• Agarose gel electrophoresis on PCR product   Michael Joiner
• Bio-Rad sequencer   Michael Joiner
• how to separate the nicked from of a plasmid from the closed form?   Zophonias Oddur Jonsson
• The end of inclusion bodies?   Loren Joseph
• Pharmacia Recombinant Phage Antibody kit?   Fourie Joubert
• Lovastatin???   K.D.Preuss
• help lambda-ZAPII-PCR??   K.D.Preuss
• parotis cDNA wanted   K.D.Preuss
• PCR and BrdU   K.M.Toellner at bham.ac.uk
• DMSO and X-gal?   MICHAEL P. KOLOTILA
• house keeping standard   KONIECZNY at FRODO.MGH.HARVARD.EDU
• unsubscribe   KORTE at UNCVX1.OIT.UNC.EDU
• RIPPED FROM TODAY'S HEADLINES: 2 month PCR?   Ron Kagan
• help lysing E. coli to purify inclusion bodies   Ron Kagan
• Mechanism of X-gal staining   Michael S. Kay
• MYCOPLASMA testing with PCR - advice sought...   Martin Kennedy
• Detecting b-gal in cultured cells ?   Martin Kennedy
• Hygromycin   Martin Kennedy
• Petri 6well/24well?   Martin Kennedy
• Hygromycin   Martin Kennedy
• pCAT Basic cut by Hind III   Martin Kennedy
• Total Digest of Proteins   Henrik Kibak
• Need a pharmaceutical salesman or biotechnican?   Konrad C. Kim
• How do you manage your working sample DNA in RAPD?   Klaus.Matthaei at ANU.EDU.AU
• Yeast Spheroplast Protocols   Klaus.Matthaei at ANU.EDU.AU
• Sequence of pTTQ18   Klaus.Matthaei at ANU.EDU.AU
• RNAse stability   David Kling
• PCR on whole yeast cells   Dave Knorr
• Eastern blotting!   Prachya Kongtawelert
• Screening glycerol-producing organisms   Charles Kosan
• Where is NCI repository?   Gopaul Kotturi
• Trouble detecting prokaryotic mRNA   Cornelius Krasel
• reusing agarose gels   Cornelius Krasel
• Removing Bind Silane from plates   Cornelius Krasel
• Removing Bind Silane from plates   Cornelius Krasel
• QIAGEN resin   Cornelius Krasel
• Problems with News archives?   Cornelius Krasel
• Eukaryotic initiation factors   Gary Krause
• Using HP scanjet as densitometer   Gary Krause
• Helper Phage missing gIII ??   Peter Kristensen
• HELP! redox potential measurement   Robert Kuhelj
• ATCC on line   Stuart Kuhstoss
• Yeast Spheroplast Protocols   LKM100F at ODUVM.BITNET
• Primer extension analysis?   LOGAN
• 2-D gel trouble   LOGAND
• Molecular Biology book   LOGAND
• Hybridisation conditions for Oligos   LOGAND
• RE. Tobac cDNA library   LOGAND
• RE. tobacco libraries   LOGAND
• oligo Southerns...HELP!   Hanson Lab
• DDRT-PCR/tiny samples   Ward Lab
• Geneclean problems   Ludwig Lab-UCSC
• Need an intro molbio book   Carlisle Landel
• FOLLOWUP: alternatives to Whatman 3MM paper for drying down gels   Carlisle Landel
• help lambda-gt11 PCR   Carlisle Landel
• Total Digest of Proteins   Stephen R. Lasky
• Using HP scanjet as densitometer   Stephen R. Lasky
• gels sticking to glassplate   Stephen R. Lasky
• Quantitating Radioactive label in DNA and RNA   Stephen R. Lasky
• DNA-contaminated PCR   Martin Leach
• oligo deprotection   Martin Leach
• oligo deprotection   Martin Leach
• PCR of episomal plasmids   Rosemary Lemons
• Bio-Rad sequencer   Rosemary Lemons
• cosmid instability   Rosemary Lemons
• Luciferase   Herbert Lensche
• Savant SpeedFuge   Martin Ligr
• cloned metalloprotease genes   Walt Lilly
• polyacrylamide-agarose gel   Lauri Lintott
• southern background problems   Lauri Lintott
• Hyb conditions   Lauri Lintott
• Hyb cond   Lauri Lintott
• T7 Promoters and exp   Lauri Lintott
• reusing agarose gels   Lauri Lintott
• bio101 fax #   Lauri Lintott
• geneclean problems   Lauri Lintott
• RNA mobility shift   Lauri Lintott
• Shareware cloning program that reads genbank feature tables?   Robert Lloyd
• Geneclean problems   Robert Lloyd
• Qiagen   Frosty Loechel
• PCR & engineer Restrict   Mark Jason Logan
• Altered Sites II- Any good?   Jeanne Loring
• leukotriene   Paul G Loubser
• Eastern blotting!   Mr. M.J. Lush
• Eastern blotting!   Mr. M.J. Lush
• Protocols for isolating large (>200kb) plasmids.   Lynda
• Differential display?   Alan Lyons
• Deletion problems   ALASTAIR MACKAY
• PCR of episomal plasmids   ALASTAIR MACKAY
• T7 promoters and expression   BARRY J MARGULIES
• Primer Labelling........HELP   MED6MHS at VAX.BIO.LEEDS.AC.UK
• ethidium bromide disposal   BRIAN PETER MERRY
• PCR on whole yeast cells   MHAMILTO at vm.ucs.UAlberta.CA
• mRNA isolation kits   TAN MIN-HAN
• Buffering Phenol   MUNGO at UVVM.UVIC.CA
• ATCC by e-mail ??   MUNGO at UVVM.UVIC.CA
• Thermocyclers   MUNGO at UVVM.UVIC.CA
• Where to get P1 phage?   Jixiang Ma
• DNA Extraction/Serum/Plasma   David MacHugh
• Test   Emma Macfarlane
• T7 promoters and expression   Emma Macfarlane
• Arabidopsis cDNA library   Emma Macfarlane
• Pcr for random mutagenesis   Emma Macfarlane
• PCR ghost bands   Emma Macfarlane
• degenerate PCR   Emma Macfarlane
• Gel dryer / pump corrosion   Paulo Magalhaes
• Oil pump / corrosion (2)   Paulo Magalhaes
• problems with phage display systems   Joseph Major
• streptogramin   Michael P. Malakhov
• streptogramin & pristinamycin & virginiamycin   Michael P. Malakhov
• Geneclean problems   Dr. D. Marazziti
• expression library   Margaret
• Thermal Cyclers   John Maris
• PCR,DNA storage for.   Monica J. Martick
• gel imaging   Viraj Master
• Lamda ExCell   Stephanie Kay Masterman
• anti adrenergic receptor antibodies   Raffaele Matteoni
• INTRONs CONSERVED!!!   Marianna Max
• ROCHE e-mail address   Marianna Max
• Pbluescript I map   Marianna Max
• Reply to "RT-PCR control primers"   John McCarrey
• Benchtop oligos: fast and cheap?   John H McDonald
• tubulin probe   McNulty, Jesse
• Papovavirus strains, antibodies & plasmids   Dan Mcleod
• Rapamycin source?   Andrew Mendelsohn
• 2-D sample prep   Mark J. Miller
• PCR for Random Mutagenesis - Help!   Steve Minchin
• PCR mutagenesis   Steve Minchin
• Protease Factor Xa ?   Steve Minchin
• T7 promoters and expression   Laura K. Moen
• baculovirus expression kits   Sean David Moore
• slot-blot advise   Sean David Moore
• **ATTENTION** +4degree C P33 isotope **INFORMATION WANTED**   Sean David Moore
• Alkaline lysis DNA prep   Sean David Moore
• WANTED- Bombproof Anchor PC   Sean David Moore
• quantifying of nucl. acids   Sean David Moore
• purify PCR products using Wizard Prep (Promega)   Sean David Moore
• QIAGEN resin   Sean David Moore
• ethidium bromide disposal   Sean David Moore
• Removing Bind Silane from plates   Sean David Moore
• bizzare digestion problem   Sean David Moore
• Eastern blotting!   Sean David Moore
• recycling pippete-tips?   Sean David Moore
• Cutting plasmid inside inverted repeats?   Shahram Mori
• How do you manage your working sample DNA in RAPD?   Shahram Mori
• RNase inhibitor   Shahram Mori
• DNA PAGE EtBr query.   Shahram Mori
• HELP! Odd Gel Pattern!!   Shahram Mori
• RNAse stability   Shahram Mori
• Cyanobacterial Promoters   Shahram Mori
• Re RT-PCR Problem****   Shahram Mori
• Pbluescript I map   Shahram Mori
• PCR & Exonuclease activity   Shahram Mori
• Nusieve Agarose who?*******   Shahram Mori
• Can a polyclonal AB distinquish between two very similar proteins   Shahram Mori
• Prok. vector x studying 5'UTR (HELP)   Shahram Mori
• Nusieve Agarose who?*******   Shahram Mori
• Translated Alu?   Mr. G. Morley
• Translated Alu?   Mr. G. Morley
• Translated Alu?   Mr. G. Morley
• Slanted Bands on Agarose Gels   Mike Morris
• Antibodies to G-protein beta subunits   Nicholas J. Morris
• oligo deprotection   Paul Morrison
• Mol. Dyn. new DNA seq. robot   Paul Morrison
• multiple antigenic peptides - MALDI   Paul Morrison
• Weird kinased oligo pattern...   G K L Munro
• Can anyone recommend a White-light light box?   Peter M. Muriana
• PCR incorp. efficiency of biotin-dUTP vs. biotin-dNTPs???   Peter M. Muriana
• Agarose gel electrophoresis on PCR product   Bernard Murray
• Inducible eukaryotic expression vector ?   Bernard Murray
• Qiagen   Bernard Murray
• hygromycin phosphotransferase   Bernard Murray
• Recommendations for bacterial hosts for "awkward" DNA   Bernard Murray
• insoluble DNA pellet after Qiagen Tip20   Bernard Murray
• using ABTS with HRP-conj for immunoscreening of lambda library   Bernard Murray
• polyquaternium   Michael L. Murray
• Transfection of Sf9/Sf21 Cells   Garry Myers
• Bio101 Fax Number?   Garry Myers
• Amount of RNase in tissues   NA
• Hints for PCR of A/T-rich sequences?   NA
• Linker interferes w Transformation?   Richard Near
• About Compressions with Sequencing   Richard Near
• oligo deprotection   Chia Jin Ngee
• Glacial Acetic Acid   White Nick
• Probing PAGE   White Nick
• Multiplexing PCR   White Nick
• Equilibrium Dialysis? Apparatus/method?   Darren Nickerson
• nitrosoguanidine   Jarmo Niemi
• b-Gal   Garry P. Nolan
• Helper Phage missing gIII ??   Chris Noren
• Ligase Chain Reaction   O'Donnell, Kevin
• patent for SDA and LCR   Kevin O'Donnell
• Test - Ignore   M.D. O'Leary
• Returned mail: User unknown (fwd)   Philip OBrien
• M2-PK or M1-PK antibody?   Jin Kar Ong
• Pbluescript I map   Francis Ouellette
• Long RT-PCR   Jim Owens
• Buffering Phenol   Jim Owens
• Buffering Phenol: The Light Dawns   Jim Owens
• Buffering Phenol   Jim Owens
• Buffering Phenol   Jim Owens
• Gel dryer / pump corrosion -   Jim Owens
• recycling pippete-tips?   Jim Owens
• Gradient Sequencing Gels   Bengt Oxelman
• Help: no transformants using BaculoGold   ANDY PHILLIPS
• Nuseive Agarose who?   JOHN PROUDMAN
• custom antibodies in chickens   JOHN PROUDMAN
• High MWt Markers   Chanpen Wiwat - PYMI
• Protocols for isolating large (>200kb) plasmids.   Chanpen Wiwat - PYMI
• Arabidopsis cDNA library   Parin
• RNA extraction from bulbs   Mick Partis
• Cloning a PCR product   Cam Patterson
• in vivo protein expression from pBluescript   Suzanne Patterson
• competitive PCR   Mark Pavlick
• competitive PCR   Mark Pavlick
• competitive PCR   Mark Pavlick
• Azotobacter gene library   Pedro
• Densitometry Software for windows or dos   Anthony J. Persechini
• pcr of ancient DNA (birds)   Petur Henry Petersen
• recycling pippete-tips?   Petur Henry Petersen
• recycling pippete-tips?   James N Petitte
• Request: human beta-polymerase   Sean Michael Philpott
• AU1 tag   Belhumeur Pierre
• Prestaining proteins   T. S. Pillay
• hamster c-myc antibodies   Geoff Pinchbeck
• (none)   Geoffrey George Pinchbeck
• sec PCR after ddPCR   A van der Plas
• automated sequencing and PCR products   Michael Poidinger
• PCR on whole yeast cells   Bert Popping
• Lovastatin   Bert Popping
• Dyes and DNA PAGE   Bert Popping
• Dyes and DNA PAGE   Bert Popping
• ATCC ON-LINE   Bert Popping
• Alkaline lysis DNA prep   Bert Popping
• Protein PCR??   Michael Powell
• Protein PCR   Michael Powell
• Protein PCR??   Michael Powell
• Can a polyclonal AB distinquish between two very similar proteins   Mike Preston
• DNA PAGE EtBr query.   Robert Preston
• re 10X TBE, dust, etc.   Robert Preston
• re dyes and DNA PAGE   Robert Preston
• Pfu-spiked cycle sequencing   Robert Preston
• Pfu-spiked cycle sequencing   Robert Preston
• PCR on whole yeast cells (fwd)   Robert Preston
• Quenching FITC (fwd)   Robert Preston
• Black DNA from earthworms   Robert Preston
• re detergent removal   Robert Preston
• Regs for mailing DNA/prions   Robert Preston
• PCR ghost bands   Robert Preston
• DNAse I shotgun MnCl2 (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• OXYGEN MEASUREMENT, Help! (fwd)   Robert Preston
• vaccinia- summary   Przemko
• vaccinia-summary   Przemko
• Amersham e-mail   RSCOTT
• Amersham e-mail   RSCOTT
• Eastern blotting!   Laurence T. Reiter
• Tth 111 II   Laurence T. Reiter
• Pichia expression   David I Resnick
• Shareware cloning program that reads genbank feature tables?   Peter Rice
• Appropriate distance b2t rib binding site & ATG?   Paul Riggs
• Optimal E Coli strains for MBP expression   Paul Riggs
• Nusieve Agarose who?*******   Paul Riggs
• >using an HP scanner/densitometer   Anne Skaja Robinson
• Microplate fluorometer favourites?   Ronald.Berry
• Gas-phase Peptide Sequencer sought   Ronald.Berry
• oligo deprotection   Dr. A. Rosenthal
• SDS-PAGE-resistant protein complexes   Alexander P Rostovtsev
• recycling pippete-tips?   Alexander P Rostovtsev
• Can a polyclonal AB distinquish between two very similar proteins   Alexander P Rostovtsev
• Drying gels w/o vacuum?   Alexander P Rostovtsev
• Inducible eukaryotic expression vector ?   Gary Rudnick
• gels sticking to glassplate   Kristen Ruebling
• Plasmid drawing program for Windows   Thomas M. Rupp
• ethidium bromide disposal   TROY PATRICK SAULNIER
• Address needed: Avanti Polar Lipids   SCHMID at Butler.EDU
• immunoscreening of cDNA library with ECL   SHAHID
• Expression in lambda-gt11   NEVIL J. SINGH
• nitrosoguanidine   SLF
• How stable is proteinase K?   Sunee Kertbundit - ST
• Pbluescript I map   Nelson Salazar
• Millipore "EXPIDITE" vs APPLIED BIOSYSTEMS 392   Roland J Saldanha
• reusing agarose gels   Diana Sammataro
• ethidium bromide disposal   Troy Saulnier
• Petri 6well/24well?   Jill Schaller
• WANTED- Bombproof Anchor PC   Steve Schramm
• WANTED- Bombproof Anchor PC   Steve Schramm
• Antibodies against ECM   Gerd Schreiter
• Autoclaving DNA and contamination   Scrimshaw, Brian J
• RNA secondary structure database search   Eddy Sean
• Taq Extender   Dave Segal
• Help: Isolation of a 80kb plasmid   Volker Seibert
• Surf Blot Experience   Hank Seifert
• CAT + Luciferase same buffer   Jorge L. Sepulveda
• PCR insitu hybridization   Sushrut Shah
• (fwd) PCR insitu hybridization   Sushrut Shah
• PCR insitu hybridization   Sushrut Shah
• T4 kinase labelling efficiency   Shauna R. Brummet, Ph.d.
• WANTED- Bombproof Anchor PC   Greg Shen
• DMSO and X-gal?   Sandy Silverman
• Help retrieving "genpept" references   Andrew Simmonds
• Hints for PCR of A/T-rich sequences?   Manuel Simon
• electroporation cuvette   Manuel Simon
• pET, lysE, lysS, bacterial expression questions   Manuel Simon
• Buffering Phenol   Manuel Simon
• Holograms from confocal img   Madhu Singh
• RNA isolation request   Andrea M Skantar
• TCA on nucleic acid   Drew Smith
• Wanted monoclonal and polyclonal antibodies against HCV   Oleg V. Sokur
• Wanted monolconal and polyclonal antibodies against HCV.   Oleg V. Sokur
• PCR ghost bands   Oliver Sorgenfrei
• RT enzyme inhibited by NP-40 and Tw20 ???   Pavel Sova
• Sequencing length with ABI sequencer   J. David Spafford
• Intron conservation   Mart Speek
• INTRONs CONSERVED!!!   Mart Speek
• baculo expression: aggregation problems   Stephan Spencer
• Hygromycin   Jamie Stanek
• purify PCR products using Wizard Prep (Promega)   Ed Stephenson
• HELP: Best Nested Deletion Kit/Method   Jason Stewart
• formaldehyde in RNA gel   B Stickney
• Used Equipment   James Stiehr
• Surf Blot Experience   James Stiehr
• Returned mail: User unknown (fwd)   John Stiles
• Quantitating Radioactive label in DNA and RNA   John Stiles
• magnetic latex ?   Ed Stokes
• antibodies to chemicals ?   Ed Stokes
• Agarose gel electrophoresis on PCR product   L. Sumoy
• 3 & 5' Overhangs...   L. Sumoy
• Buffering Phenol   L. Sumoy
• gels sticking to glassplate   L. Sumoy
• Geneclean problems   James S. Sutcliffe
• Geneclean problems   Bruce E. Taillon
• FOLLOWUP: alternatives to Whatman 3MM paper for drying down gels   Song Tan
• multiple antigenic peptides - MALDI   Lorne Taylor
• HIS TAG and antibody affinity purification   Tom Thatcher
• ?Mouse breeding database   Tom Thatcher
• gels sticking to glassplate   Peter Thorén
• oligo deprotection   Graham Threadgill
• bacterial killing   Michael J. Tino
• Quenching FITC   Michael J. Tino
• Help with large SDS-PAGE gels   Gus Trautweiler
• SDS-PAGE-resistant protein complexes   Paul J Travers
• Can a polyclonal AB distinquish between two very similar proteins   Paul J Travers
• Mammalian cells for high efficiency transfection   Bruce R. Troen
• Help ! Methyl Mercury Hydroxide   Bradley Turner
• Moravek Biochemicals address   U196CFA at CSI.IT
• Equilibrium dialysis ? Apparatus/method   U196CFA at CSI.IT
• PKA phosphorylation in vitro   Peter Uetz
• EGF receptor prep??   VOSS at AIMP1.UNA.AC.AT
• Suggestions/comments on Exonuclease III deletions?   Jean Valjean
• TCA on nucleic acid   Frank Vari
• MORE on Non ppt 10x TBE (for the pedantic amongst you   I.B. Vipond
• Advice re: Stratagene VAGE Apparatus   Dick Vogt
• Transgenic constructs - HELP!!!   Dominic Voon
• Help needed to re-cycle QIAGEN <plasmid> tips   Dominic Voon
• Help ! Methyl Mercury Hydroxide   Dominic Voon
• Water soluble trivalent cation   WANDERS at INDYVAX.IUPUI.EDU
• Pseudo fpu   WANDERS at INDYVAX.IUPUI.EDU
• quantifying of nucl. acids   HYNEK WICHTERLE
• conc.of NA - summary   HYNEK WICHTERLE
• Protease Factor Xa ?   HYNEK WICHTERLE
• Protease Factor Xa ?   HYNEK WICHTERLE
• problems with PCR   WMGDDM
• books on PCR   WMGDDM
• DNA PAGE EtBr query.   Michael van Waes
• Overcoming Sequencing Secondary Structure   Michael van Waes
• help on direct sequencing of PCR products   FJ van der Wal
• Amersham e-mail   R.G. Walters
• Updated MicroGenie?   Shaojiu Wang
• Need an intro molbio book   Shiao Y. Wang
• Radiolabeled probes by PCR?   Shiao Y. Wang
• Total phosphate assay?   Shiao Y. Wang
• Buffering Phenol: The Light Dawns   Shiao Y. Wang
• PCR & Exonuclease activity   Shiao Y. Wang
• Gel dryer / pump corrosion -   Shiao Y. Wang
• help lambda-gt11 PCR   Shiao Y. Wang
• PCR & Exonuclease activity   Shiao Y. Wang
• mRNA isolation from total RNA Kits (NEW)   Shiao Y. Wang
• Zoo blots   John Watson
• DNAse I shotgun MnCl2   P Watson
• The end of inclusion bodies?   Christoph Weber
• Glacial Acetic Acid   James R. Weber
• Glacial Acetic Acid   James R. Weber
• How necessary to prevent self-ligation using CIP?   Helge Weissig
• Can I successfully blunt it?   Helge Weissig
• mRNA purification   Helge Weissig
• RNase inhibitor   Helge Weissig
• Black DNA from earthworms   Patrick Weix
• Auto-DNAprep machines   Roger Wiegand
• Autoclaving DNA and contamination   Roger Wiegand
• HELP: Screening bact.-colonies with Ab?   Troels Wind
• Nusieve Agarose who?*******   Neil Winegarden
• gels sticking to glassplate   Neil Winegarden
• Isolating monocytes from peripherla blood?   Donald Chun Kit Wong
• pDR540 sequence   Surasakdi Wongratanacheewin
• Thermal cycle from Pharmacia   Surasakdi Wongratanacheewin
• Eastern blotting!   Jim Woodgett
• T7 promoters and expression   Jim Woodgett
• RIPPED FROM TODAY'S HEADLINES: 2 month PCR?   Jim Woodgett
• WANTED HP Temperature Station   the X-Man
• Shareware cloning program that reads genbank feature tables?   Nanfel Xu
• West blotting   S. Xu
• 24-well petridish, again.   Yamagat
• Petri 6well/24well?   Yamagata （山形）
• +4C P33 isotope   Fariborz Yazdani
• **ATTENTION** +4degree C P33 isotope **INFORMATION WANTED**   Fariborz Yazdani
• T7 RNA polymerase inhibitor   Yim, Harry M'bio
• T7 RNA polymerase inhibitor   Yim, Harry M'bio
• Trouble detecting prokaryotic mRNA   Karen L. Yoas
• RNA mobility shift   Christopher B. Yohn
• Drying gels w/o vacuum?   Dongxian Yue
• TCA on nucleic acid   Dongxian Yue
• DNA extraction from sputum   Zainul Zain
• LumiPhos for Westerns?   Piotr Zimniak
• non-radioactive translation kits   Frances Hannan Zoology
• Problems with News archives?   Frances Hannan Zoology
• sick JM109's   Frances Hannan Zoology
• color pictures of silver-stained gels   Christian Zwieb
• RNA secondary structure database search   Christian Zwieb
• PCR on whole yeast--reference?   alvin at UNITY.NCSU.EDU
• books on PCR   eric c. anderson
• YAC vectors w/AscI site?   andre at mpimg-berlin-dahlem.mpg.de
• baculovirus expression kits   ashendel at aclcb.purdue.edu
• purify PCR products using Wizard Prep (Promega)   ashendel at aclcb.purdue.edu
• Advice re: lyophilizers requested   axelsen
• Quick and cheap +/- PCR screen?   bagley at fauna.ucdavis.edu
• Newsgroup bionet.molbio.methds-reagnts, Articles 15429-15458-antibodies to Rabs   bknoll at bcm.tmc.edu
• Need mammalian luciferase vector   books at mcvax2.d48.lilly.com
• pUC18 as expression vector   botn056 at csc.canterbury.ac.nz
• Slanted Bands on Agarose Gels   botn056 at csc.canterbury.ac.nz
• HELP! Odd Gel Pattern!!   brunker at delphi.com
• RIPPED FROM TODAY'S HEADLINES: 2 month PCR?   brunker at delphi.com
• Sizing microsatellites. Is there a true answer?   carlos
• Thermal Cyclers   carlos
• Hamster c-myc   chelbing at ACS.UCALGARY.CA
• how to separate cccDNA from nicked circular plasmid   churchillj at HAL.HAHNEMANN.EDU
• pCAT Basic cut by Hind III   cjharrod at waikato.ac.nz
• Alkaline lysis DNA prep   craig.rowlands at csb.ki.se
• Hygromycin   dalehn at facstaff.wisc.edu
• Buffering Phenol(sounds like 'Kit Wars')   dallas at mcvax4.d48.lilly.com
• "Standard" housekeeping gene   darin at lenti.med.umn.edu
• MYCOPLASMA testing with PCR - advice sought...   davis at wehi.edu.au
• b-Gal   dh7y at pop.th-darmstadt.de
• Coli Genetic Stock Center - on-line??   dkatz at earth.doane.edu
• location of Biosafety Manual?   dkatz at earth.doane.edu
• Undergrad. computer/teaching use help   dkatz at earth.doane.edu
• Quick and cheap +/- PCR screen?   dkim at unm.edu
• Surf Blot Experience   dkim at unm.edu
• dissection and RNA extraction   dkim at unm.edu
• PCR machine patents   dkim at unm.edu
• Eastern blotting!   dkim at unm.edu
• TCA on nucleic acid   dkim at unm.edu
• Cloning a PCR product   dkim at unm.edu
• Differential display?   dkim at unm.edu
• purify PCR products using Wizard Prep (Promega)   dkim at unm.edu
• Isolation of Total RNA from just about anything   dkim at unm.edu
• Hyg, Neo, and $$   down-n-out
• Nuclear isolation   dpuumala at flash.LakeheadU.Ca
• Remove CH3CN and other solvent from small RNA (4mer)   dxy at iastate.edu
• INTRONs CONSERVED!!!   norm eberhardt
• Anything other than TLC's for CAT assays?   batu erman
• KEM-EN-TEC CREAM imaging system   exb0431 at csdvax.csd.unsw.edu.au
• magnetic latex/polysciences reply   exb0431 at csdvax.csd.unsw.edu.au
• using a HP IIp scanner/densitometer   exb0431 at csdvax.csd.unsw.edu.au
• PCR ghost bands   exb0431 at csdvax.csd.unsw.edu.au
• polyacrylamide-agarose gel   futers at bpxtal.leeds.ac.uk
• Isolation of Total RNA from just about anything   fzestabr at chip.ucdavis.edu
• Help wanted. Direct sequencing Lambda   gaitken at chmeds.ac.nz
• Qiagen   gc
• Qiagen   gc
• Glowing Blue Spots   gc
• Help retrieving "genpept" references   gc
• LUCIFERASE woes   gc
• hamster-specific c-myc antibodies   ggpinchb at ACS.UCALGARY.CA
• Protocols for isolating large (>200kb) plasmids.   goyal at mbcl.rutgers.edu
• Quenching FITC   m griep
• Cloning 12-15kb DNA   gritsun at molbiol.ox.ac.uk
• ssDNA-Exo3 vs Taq??   gubbinse at rand
• Radiolabeled probes by PCR?   gvacano at eagle.wesleyan.edu
• How stable is proteinase K?   Domingos henrique
• Screen a library for amidase?   hetherington at mbcf.stjude.org
• Long RT-PCR   hetherington at mbcf.stjude.org
• HELP IP3/PLC ASSAY   hhmq at umich.edu
• Auto-DNAprep machines   hollingswor at immunex.com
• protein express/purification   irlbeck at uncvx1.oit.unc.edu
• pET, lysE, lysS, bacterial expression questions   jfh
• help lysing E. coli to purify inclusion bodies   jfh
• ECL detection system   jgill at kean.ucs.mun.ca
• Need Help w/ Myco   jkeithan_eff at opal.tufts.edu
• Proteins Wanted   brian h. johnson
• Proteins wanted II   brian h. johnson
• plasmids containing amber mutations in selectable markers   jpcd0 at mole.bio.cam.ac.uk
• neighboring/pcr   jpetree at opal.tufts.edu
• Direct RNA sequencing!   jr at dna.bio.warwick.ac.uk
• Epitope mapping technique?   kjwile at indyunix.iupui.edu
• oligo Southerns...HELP!   kmorris1 at opal.tufts.edu
• PCR final hold temp. article???   ktgeiss at miavx1.acs.muohio.edu
• mRNA isolation kits   Rich Maki's lab
• Isolation of Total RNA from just about anything   Winoto lab
• Cloning 12-15kb DNA   Winoto lab
• Transfection efficiency for CAT assays   Winoto lab
• dissection and RNA extraction   Winoto lab
• Glowing Blue Spots   Winoto lab
• Electroporator recommendation request   Winoto lab
• Ligation of PvuI site   Winoto lab
• RNA isolation request   Winoto lab
• Sequence of pTTQ18   Winoto lab
• purify PCR products using Wizard Prep (Promega)   Winoto lab
• Thermal Cyclers   Winoto lab
• bizzare digestion problem   Winoto lab
• pCAT Basic cut by Hind III   Winoto lab
• problems with PCR   Winoto lab
• Need mammalian luciferase vector   Winoto lab
• antibodies to chemicals ?   Winoto lab
• Isolation of Total RNA from just about anything   Winoto lab
• purify PCR products using Wizard Prep (Promega)   Winoto lab
• helix-turn-helix   lgmm at cismibm.univ-lyon1.fr
• Protocol needed for raising Ab. to overexpressed proteins   liyer at bioch.tamu.edu
• Glacial Acetic Acid   lmullen at eye1.eye.ufl.edu
• Qiagen oligotex mRNA tissue   Bulletin board login
• Ligation of PvuI site   Bulletin board login
• Kanamycin inhibits EcoR1?!   ???? maria
• Phage display in Israel   markmano at bcvms.bc.edu
• Cross-linking   mazella
• Help needed to re-cycle QIAGEN   mccallo at rand
• The end of inclusion bodies?   mccallo at rand
• Black DNA from earthworms   mccallo at rand
• ??? Vector to clone a gene by complemntation of E. coli mutant   mcgraw at BSCR.UGA.EDU
• Long PCR smear   mhollowa at epo.som.sunysb.edu
• T-antigen binding sites in mammalian genome   mhollowa at epo.som.sunysb.edu
• insoluble DNA pellet after Qiagen Tip20   mhollowa at epo.som.sunysb.edu
• PCR primers for ribosomal RNA   mohanish at pharmdec.wustl.edu
• Glacial Acetic Acid   neale at mbcf.stjude.org
• Qiagen   neale at mbcf.stjude.org
• Electroporator recommendation request   neale at mbcf.stjude.org
• Problems with News archives?   neale at mbcf.stjude.org
• Another NEWS archive search site   neale at mbcf.stjude.org
• lambda gt6   nohmi at nihs
• problems with PCR   stephen nurrish
• RNA second. structure database   otello at sc2a.unige.ch
• tRNA search (HELP)   otello at sc2a.unige.ch
• Prok. vector x studying 5'UTR (HELP)   otello at sc2a.unige.ch
• Plasmid drawing program for Windows   pATEL_P
• EMBL-3 gDNA library construction   pdearden
• Elutip-D v. Elu-Quick   phoward at SN1.CVM.NCSU.EDU
• About Compressions with Sequencing   pmiguel at bilbo.bio.purdue.edu
• Sequencing with Taq Polymerase   pmiguel at bilbo.bio.purdue.edu
• Transfection of Sf9/Sf21 Cells   polk at molbiol.ox.ac.uk
• Transfection of Sf9/Sf21 Cells   polk at molbiol.ox.ac.uk
• antibodies search   ptc at ncl.ernet.in
• plant kinase probes   ptc at ncl.ernet.in
• Cre recombinase clones?   kenneth garson cancer research
• Cre recombinase clones (with text?)?   kenneth garson cancer research
• Amount of RNase in tissues   rhubner at molbiol.ox.ac.uk
• Immunoblotting Kits   riadl3261 at cobra.uni.edu
• using ABTS with HRP-conj for immunoscreening of lambda library   robert at chironex.jcu.edu.au
• detergent removal help!   scott_basehore at stratagene.com
• Detecting b-gal in cultured cells ?   simmenlab at animal.ufl.edu
• Black DNA from earthworms   sjames at miu.edu
• (none)   sudhanshu at nii.ernet.in
• miniprep blues !   sudhanshu at nii.ernet.in
• Buffering Phenol   szcooley at chip.ucdavis.edu
• test   szuratsu at chip.ucdavis.edu
• PCR don't work   cilia jonas t2814
• how to separate the nicked from of a plasmid from the closed form?   t_rolden at icrf.icnet.uk
• test(Please ignore it)   takeuchi
• Problems with News archives? IUBio disk failures again   tan at aeolus.vmsmail.ethz.ch
• Very large Plasmid Preps   tan at aeolus.vmsmail.ethz.ch
• Isolation of RNA from just about anything   terry_syminis at FMC.COM
• Transfection efficiency for CAT assays   tsichlis at anaximander.rm.fccc.edu
• ethidium bromide disposal   vaidyaa at HAL.HAHNEMANN.EDU
• ethidium bromide disposal   vaidyaa at HAL.HAHNEMANN.EDU
• Prep of M13 RF DNA   vldnaseq at hkucc.hku.hk
• Anything other than TLC's for CAT assays?   walling at wmvx.lvs.dupont.com
• leukotriene   walling at wmvx.lvs.dupont.com
• HELP IP3/PLC ASSAY   walling at wmvx.lvs.dupont.com
• OXYGEN MEASUREMENT, Help!   vitor warwar
• Yeast Spheroplast Protocols   whitman at arris.com
• Thermal Cycler   wochniak at SGGW.SGGW.WAW.PL
• Thermal Cycler   wochniak at SGGW.SGGW.WAW.PL
• Looking for MJ Research address   wochniak at SGGW.SGGW.WAW.PL
• Looking for MJ Research address   wochniak at SGGW.SGGW.WAW.PL
• ethidium bromide disposal   ww40
• RNase inhibitor   wzhao at phy.bcm.tmc.edu
• Eastern blotting!   David Martin x3175
• Help retrieving "genpept" references   yagi2 at scripps.edu
• (none)   zeilerk at TCPLINK.NREL.GOV

Last message date: Thu Jun 30 22:52:31 EST 1994
Archived on: Sun Mar 6 20:09:28 EST 2005

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