southern blot

Kieron Des kierondes at
Tue Mar 1 13:25:02 EST 1994

Baking will immobilize DNA on a nylon membrane. You dont need a vacuum oven
because nylon membranes do not have a high flash point the same way that
nitrocellulose (AKA guncotton) does. The baking will hold the target DNA just
about as well as UV crosslinking. Biotechniques had an article about this a
while back. I think the lead author was Sandra A. Nierzwicki-Bauer.

Tell your friend to go for it

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