a.hanly at cheque.uq.oz.au
Wed Mar 2 01:15:43 EST 1994
On Tue, 1 Mar 1994 17:18:22 GMT,
Stephen Morris <smorris at pharma1.pharma.mcgill.ca> wrote:
>Hi, Does anyone know of a recipe for hybidization cocktail that is quicker
>than the standard formamide - dextran mixture? I've been using
>Formamide/20xssc/2M tris/100x denharts/water/10% sds/50% dextran
>(92/48/1/2/11/18/40) and it's pretty slow.
I've recently started using the Amersham rapid hybridisation buffer and have
had good results (so far ... touch wood).
Extract from product specification .....
"Rapid-hyb buffer is tested ... to ensure that a single copy gene can be
detected in human genomic DNA blots .... following a 2 hour hybridisation
at 65 deg... For many applications a 1 hr hybridisation is sufficient "
The protocol is optimised for Hybond-N+ but I'm using Hybond-N.
Use with nitrocellulose or PVDF membrane is not recommended by the
Can be used for radio-labelled DNA, RNA or oligo probes.
I've used it for Northerns and Southerns with random-labelled DNA probe.
Amersham cat No: 1635 - 125ml
1636 - 500ml
No Affiliatios with etc etc....
More information about the Methods