Nonradioactive vs. radiolabelled probes

Marion Freistadt mfreis at lsumc.edu
Wed Mar 2 14:53:51 EST 1994


Has anyone compared the sensitivity of non vs. radiolabelled probes in
southern blots, all else being equal?  We are having difficulty detecting a
single copy sequence used the Genius kit; but we know the gene is there by
PCR.  The positive control in the southern (plasmid DNA) works, although we
have not yet titrated the sensitivity, hoping it wouldn't be necessary.  I
was thinking maybe I should go back to 32P.  Comments?




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