A problem in metabolic radiolabelling

Hinayana Bawagan bawagan at umdnj.edu
Wed Mar 2 19:58:25 EST 1994


Dear netters,

     Here's the situation.  I wish to see if my expression vector produce the
foreign proteins from the inserted cDNA.  Since I don't have any antibody at
the moment, I have to contend of course with all the radiolabelled proteins
in an SDS PAGE profile.  The proteins are also structural so I don't have an
assay for activity.  I am planning to do a Northern to check for message
quantity.  But how do I visualize in the best possible way the foreign
proteins?



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