In vitro Transcription/Translation

37_527 at uwovax.uwo.ca 37_527 at uwovax.uwo.ca
Wed Mar 2 15:34:53 EST 1994


    Hi;
           
          I'm looking for an in vitro transcription/translation 
kit. I like to express  Ig H and L genes. I would like to lable each peptide
with a differnt lable(35S Met & 14C Leu for example). I would appretiate any
comment on what kit? what lables? and IS there any preferance for having a 
eu or prokaryotic system?
          Finally I know promega has a TnT coupled reticulocyte lysate system,
anybody has a comment on this system?

Thanks in Advance.
Mahmoud
37_527 at uwovax.uwo.ca
.




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