M13 ssDNA-elctrphrtic species??

Dick Beeman beeman at crunch.usgmrl.ksu.edu
Thu Mar 3 06:21:42 EST 1994

Can anyone cite a reference that discusses in detail (more detail than
found in Current Protocols) the identities of the various bands seen on
agarose gels after electrophoresis of M13 single-stranded DNA (i.e. the
supernatants after spinning cells down)?  Why does the ssDNA migrate as a
doublet with constant spacing irrespective of insert size?  What are the
critical factors in getting a good yield of this doublet without excess
contamination with the common (bacterial chromosome?) band?  Thanks!!  

Dick Beeman
beeman at crunch.usgmrl.ksu.edu
"One of the advantages of being disorderly is that one is constantly making
exciting discoveries".  A. A. Milne

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