M13 ssDNA-elctrphrtic species??

Dick Beeman beeman at crunch.usgmrl.ksu.edu
Thu Mar 3 06:21:42 EST 1994


Can anyone cite a reference that discusses in detail (more detail than
found in Current Protocols) the identities of the various bands seen on
agarose gels after electrophoresis of M13 single-stranded DNA (i.e. the
supernatants after spinning cells down)?  Why does the ssDNA migrate as a
doublet with constant spacing irrespective of insert size?  What are the
critical factors in getting a good yield of this doublet without excess
contamination with the common (bacterial chromosome?) band?  Thanks!!  

-- 
Dick Beeman
beeman at crunch.usgmrl.ksu.edu
"One of the advantages of being disorderly is that one is constantly making
exciting discoveries".  A. A. Milne



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