metabolically labeled nucleotides

Dean R. Appling appling at utbc01.cm.utexas.edu
Thu Mar 3 10:06:02 EST 1994


We need to prepare large amounts of 13C-labeled deoxynucleotides by
metabolically labeling cells (E. coli?) with 13C-acetate (a cheap labeled
carbon source).  The labeled nucs will be prepared by enzymatic digestion
of the labeled DNA.  Does anyone know of references from the old
literature that can give us some idea of expected yields and conditions? 
References to 32P or 14C labeling would also be welcome.  Thanks.



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