Experience with BioRad Rotofor system
Andrew, Tel. +39-6-91093434
WALLACE at IRBM.IT
Thu Mar 3 09:08:10 EST 1994
-John <newittja at delphi.com> writes:
>"Steve A. Kay" <sak5y at FARADAY.CLAS.VIRGINIA.EDU> writes:
>> Has anyone had any experience with the BioRad Rotofor
>>and/or Prep Cell protein purification systems ? We are
>>considering purchasing one and would appreciate any comments.
>I tested the Rotofor once with an E. coli lysate from cells ex-
>pressing my protein at moderate levels. A MAJOR problem with
>this apparatus is that once the proteins near their pI, they
>precipitate. I tried several of the suggested workarounds, but
>I never had any success. We didn't buy the apparatus.
A student in our lab tried the apparatus and had exactly the same
problem. We didn't buy it either.
Andrew <wallace at irbm.it>
More information about the Methods