Experience with BioRad Rotofor system

Andrew, Tel. +39-6-91093434 WALLACE at IRBM.IT
Thu Mar 3 09:08:10 EST 1994


	-John  <newittja at delphi.com> writes:

>"Steve A. Kay" <sak5y at FARADAY.CLAS.VIRGINIA.EDU> writes:
 
>>Dear Netters,
>>
>>        Has anyone had any experience with the BioRad Rotofor
>>and/or Prep Cell protein purification systems ? We are
>>considering purchasing one and would appreciate any comments.
 
 
>I tested the Rotofor once with an E. coli lysate from cells ex-
>pressing my protein at moderate levels.  A MAJOR problem with
>this apparatus is that once the proteins near their pI, they
>precipitate.  I tried several of the suggested workarounds, but
>I never had any success.  We didn't buy the apparatus.
 

A student in our lab tried the apparatus and had exactly the same 
problem. We didn't buy it either.

Andrew <wallace at irbm.it>



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