A problem in metabolic radiolabelling

T. S. Pillay tpillay at ucsd.edu
Sat Mar 5 02:00:44 EST 1994


Subject: A problem in metabolic radiolabelling
From: Hinayana Bawagan, bawagan at umdnj.edu
Date: 3 Mar 94 00:58:25 GMT
In article <CM2DDE.8pt at umdnj.edu> Hinayana Bawagan, bawagan at umdnj.edu
writes:
>
>Dear netters,
>
>     Here's the situation.  I wish to see if my expression vector
produce the
>foreign proteins from the inserted cDNA.  Since I don't have any
antibody at
>the moment, I have to contend of course with all the radiolabelled
proteins
>in an SDS PAGE profile.  The proteins are also structural so I don't
have an
>assay for activity.  I am planning to do a Northern to check for message
>quantity.  But how do I visualize in the best possible way the foreign
>proteins?

You haven't made the situation very clear.  Are you talking about a
eukaryotic expression vector or not (I have assumed this).   I would
suggest 2-gel electrophoresis (IEF + SDS PAGE) comparing both transfected
and untransfected cells.  
Hope this is useful.



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