how to isolate a plasmid from Brevibacterium ammoniagenes?

aklib at aklib at
Fri Mar 4 23:54:21 EST 1994

I am posting this for a friend who does not have direct usenet access :-(.
How to isolate a large (25Kb)  low-copy (several per genome) plasmid
from Brevibacterium ammoniagenes (ATCC 6872)?
Please reply here and if possible send a copy to dusha at

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