ss-DNA-Ligation

Dorian Winter DORIAN at bch.univie.ac.at
Fri Mar 4 06:43:01 EST 1994


Hi, Netters,

We d like to ligate ss-DNA oligos to the 5 -overhang of our vector.
This happened, we need to fill up the single-strand, creating a blunt
end.
Our questions are:
1. Is this possible?
2. Which is the most suitable ligase and buffer-system?
3. Which is the polymerase of choice?

Any suggestions are welcome!

Isabella Halama and Dorian Winter
Institute of Biochemistry
University of Vienna





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