Wizard & Sequencing

[Roger Wiegand]

In article <2l5m0c$98q at usenet.INS.CWRU.Edu>, dd034 at cleveland.Freenet.Edu
(Seth Daniel Crosby) wrote:

> 
> We've been having some problems with automated (ABI) sequencing
>  of plasmid isolated with Wizard minipreps.  Any other
>  experiences?


My experience with Wizards ranges from fair to poor with a high degree of
variability. This is confirmed by the ABI tech rep. Side-by-side, Magics
were good (350-400 readable), Wizards poor (0-200 readable), and
Qiawell/Qiaprep mini's excellent (550+ readable) for sequencing the same
pBBR327-based plasmid from bugs (TG-1) grown up and aliquoted. (Done in
triplicate). The Qiagen kits are nice if you can afford them, which I can't
at any scale. The alkaline lysis/PEG pptn prep in the ABI manual is
reported to give very good sequence, next time I'll include it in my
comparison.
-- 
Thanks,
Roger

rcwieg at ccmail.monsanto.com