making antibodies to low antigenicity proteins

Ding Ming ming at ahabs.wisc.edu
Sat Mar 5 11:38:23 EST 1994


In article <2l9mko$76l at mserv1.dl.ac.uk>, cooke at univ-perp.fr (Richard Cooke)
wrote:

> Bonjour,
> 
>         A colleague of mine is looking for references or methods for
> raising antibodies against small, hydrophilic proteins. She has heard of a
> method involving binding of IgGs to the protein. Any information would be
> greatly appreciated.
If you have IgGs for this protein, why do you bother to make Ab again? :)
I think you were talking about coupling rather than binding, right?

Anyway, for those small,  hydrophilic proteins, you probably need to
conjugate them to some generic proteins like BSA, KLH or thyroglobulin.
Simply treat them as synthetic peptide haptens. Another trick is to
immunize animals with either SDS-PAGE or nitrocellulose powders if your
protocol allows you do that.

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Ding Ming
University of Wisconsin-Madison
Telephone: (608)265-3544     Fax: (608)262-7420
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