SOUTHERNS

Martin Leach leach at mbcrr.harvard.edu
Sat Mar 5 11:00:39 EST 1994


In article <1994Mar3.155442.1 at matai.vuw.ac.nz>, frances at matai.vuw.ac.nz
wrote:

> HELP!  Has anyone ever had problems caused by "reverse capillary action" when
> they are Southern Blotting.  By this I mean getting a ghost of an image from
> one gel (previously blotted using the same Southern set-up) transfered onto
> another.
> 
> I realise that the easy answer to this problem would be to use a new Southern
> Blot set-up each time, but I am interested in hearing from people who have
> experienced this phenomenon.
> 
> I'm just curious I suppose (or in need of vindication).  If you have nothing
> better to do please drop me a line.
> 
Check the intensifying screens also. I a wet blot touches these they can
get contaminated and give ghost images.

ML
-- 

.....          Martin Leach                Email:leach at mbcrr.harvard.edu 
   _|____      Dept. of Pharmacology       Phone: (617) 638-5323        
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