udbs061 at bay.cc.kcl.ac.uk
Sat Mar 5 17:12:44 EST 1994
I am having problems in staining gels using Nitroblue tetrazolium
to study isozymes eg. alcohol dehydrogenase,fumarate hydratase etc.
The major problem is I am supposed to get blue-violet bands at the regions
of my isozymes instead i get the ghostimages of the the band and my
gel get stained purplish blue. Has any body faced this problem and know the
reasons. I run native PAGE to study an array of isozymes.
Any assistance will be well appreciated
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