E. coli flg(Hag) mutants

KRUEGER, CHARLES M krueger at rigel.tamu.edu
Sat Mar 5 13:20:00 EST 1994


Greetings,
	Recently we have cloned what appears (from DNA 
sequence similarity) to be the flagellin gene from
a motile gram-negative bacterium.  The clone includes
the native promoter, RBS, start and stop sites.  I would
like to transform a flg(Hag) mutant strain of E. coli
to see if I could restore motility.  There is a number of
E. coli flg mutant strains.  Is there a particular Hag
mutant strain of E. coli that would be ideal for this
experiment (i.e., defect only in the flagellin protein).
				Thanks,
				Charles M. Krueger
				Texas A&M University
				College Station, Texas USA
				Krueger at ZEUS.tamu.edu
				72714.2240 at Compuserve.com



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