experiences with pET vectors from Novagen

Cornelius Krasel krasel at alf.biochem.mpg.de
Sat Mar 5 13:31:35 EST 1994


Bipin K Dalmia (bipin at iastate.edu) wrote:
: i'm looking for +ve and -ve experiences that you have had with the pET
: expression vectors from Novagen. more specifically with the N-terminal
: his.tag systems. i have to overexpress a vareity of proteins and i have
: chosen this system because it offers the advantage of purification in
: the presence of denaturants. most proteins overexpressed do form
: inclusion bodies don't they!

The protein we routinely overexpress with a pET vector is phosducin, and
it does not form inclusion bodies. If you don't know how to purify your
proteins or if the purification is tedious, tagging is definitely helpful.

--Cornelius.

--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795 */
/* "People are DNA's way of making more DNA." (Edward O. Wilson, 1975)    */



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