experiences with pET vectors from Novagen

Anne Savitt asavitt at sunysb.edu
Sun Mar 6 14:27:04 EST 1994


Re: experiences with pET vectors from Novagen

I first cloned my protein - a small DNA binding protein - into a GST
fusion for expression and ended up with lots of degradation or truncated
proteins, even in a protease-deficient bacterial strain.  I have now put
it into a pET vector with a His-tag and express it in BL21(DE3)/pLysS and
get good expression with single step purification on their affinity
matrix with no degradation.  I!m happy.

Anne Savitt
Department of Microbiology
SUNY at Stony Brook
Stony Brook, NY
email:  asavitt at sunysb.edu



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