Redivue radionucleotides from Amersham
a.hanly at cheque.uq.oz.au
Tue Mar 8 00:27:06 EST 1994
On 7 Mar 1994 16:28:52 -0000,
WMGDDM <WMGDDM at CARDIFF.AC.UK> wrote:
> Recently our lab has switched to using the new Redivue
>radionucleotides from Amersham.(For those who aren't
>familiar with this product it apparently is nucleotide
>containing a violet dye that allows the user to 'see' where
>the radioactivity is).
> Anyway since using the new product we have been getting
>poor results with hot-PCR reactions that previously were
>OK. This is also true for other reactions eg. sequencing,
> The big question is has anyone out there had similar
>problems to us or are we just going thru a bad patch ;-)
>Any help or info would be gratefully appreciated even if
>its just to corroborate our findings.
I've yet to use the redivue for PCR or sequencing but have used the
P32 dCTP for northern and Southern hybridisations without problems.
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