Redivue radionucleotides from Amersham

Allison Hanly a.hanly at cheque.uq.oz.au
Tue Mar 8 00:27:06 EST 1994


On 7 Mar 1994 16:28:52 -0000, 
WMGDDM  <WMGDDM at CARDIFF.AC.UK> wrote:

>    Recently our lab has switched to using the new Redivue 
>radionucleotides from Amersham.(For those who aren't 
>familiar with this product it apparently is nucleotide 
>containing a violet dye that allows the user to 'see' where 
>the radioactivity is).
>    Anyway since using the new product we have been getting 
>poor results with hot-PCR reactions that previously were 
>OK. This is also true for other reactions eg. sequencing, 
>etc.
>    The big question is has anyone out there had similar 
>problems to us or are we just going thru a bad patch ;-)
>Any help or info would be gratefully appreciated even if 
>its just to corroborate our findings.

Hi,
    I've yet to use the redivue for PCR or sequencing but have used the 
P32 dCTP for northern and Southern hybridisations without problems.

Allison



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