Tissue Damage Using In Situ/PCR?

[William Anderson]

Hello,
	I am contemplating using one of the new PCR machines that 
accomodate slides to generate the probe on the slide itself, but am 
concerned about morphological degradation within tissue sections exposed 
to repeated heat/cool cycles.  I usually use 12um frozen sections that 
are lightly fixed just prior to use in ice cold 4% paraformaldehyde for 5 
minutes.  Does anyone know if my tissue is going to be turned into Swiss 
cheese or if I can alleviate any problems by fixing the tissue harder?  
Any advice is welcome!

Will Anderson
wanderso at u.washington.edu