PcAb Elution problems
matiasm at rand
matiasm at rand
Mon Mar 7 10:26:14 EST 1994
I am having problems eluting off PcAb from an antigen affinity column.
I have tried eluting the PcAb with glycine, pH 2.6, and with 3 Molar MgCl2,
and combinations thereof. I have also tried electroeluting, but so far with
no apparent success. Can anyone give me anymore suggestions in eluting off
this seemingly high-affinity "mother of all antibodies" ?
Matt at Abbott Labs
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