PcAb Elution problems

matiasm at rand matiasm at rand
Mon Mar 7 10:26:14 EST 1994

I am having problems eluting off PcAb from an antigen affinity column.
I have tried eluting the PcAb with glycine, pH 2.6, and with 3 Molar MgCl2,
and combinations thereof.  I have also tried electroeluting, but so far with
no apparent success.  Can anyone give me anymore suggestions in eluting off
this seemingly high-affinity "mother of all antibodies" ?

Matt at Abbott Labs

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