Plasmid extraction

Shahram Mori smori at
Mon Mar 7 19:23:37 EST 1994

micjms at wrote:
: Is there anyone out there that can give me some advice on plasmid extraction
: protocols of large plasmids i.e 30 kb and over.

: I await to hear some type of response..

: Joanne

Dear Joanne,
I have been using the regular Mini-prep procedure of Maniatis. My cosmid has
inserts between 20-30K in it. I haven't had any problems with it.
Good Luck
Shahram Mori
Program in Molecular Biology 
Dept. of Chemistry
Box 3c   NMSU Las Cruces NM
 _\  /_
 \_  _/

More information about the Methods mailing list