Plasmid extraction

Shahram Mori smori at nmsu.edu
Mon Mar 7 19:23:37 EST 1994


micjms at lure.latrobe.edu.au wrote:
: Is there anyone out there that can give me some advice on plasmid extraction
: protocols of large plasmids i.e 30 kb and over.

: I await to hear some type of response..

: Joanne

Dear Joanne,
I have been using the regular Mini-prep procedure of Maniatis. My cosmid has
inserts between 20-30K in it. I haven't had any problems with it.
Good Luck
Shahram Mori
Program in Molecular Biology 
Dept. of Chemistry
Box 3c   NMSU Las Cruces NM
88003
   
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