transfection in suspension culture

[Therese Ann Dietlin]

Dear Netters,

We want to transfect a cell line that is non-adherent.  We are planning to prep the plasmid DNA with DOTAP and do the transfection in suspension.  We have not done this before and would appreciate any helpful information that others might have to offer.

Please post or email to tdietlin at zygote.hsc.usc.edu

Thanks in advance and thanks to dbates for calling my attention to my earlier failed attempt to post this.

Therese Dietlin