Who is the first dicover PCR technique?

[Wasun Chantratita]

Dear Netters,

	It may correct to say that Dr. Kary B. Mullis, a research
scientist at the Perkin-Elmer Cetus Corporation in April 1983 was
initially developed a modern PCR technology. However, I recently read an
article described the first PCR experiment was actually performed by Dr.
Kjell Kleppe, a postdoctoral fellow in Dr.  Khorana 's laboratory. This
experiment was a reaction involving two cycles of amplification of a 30
nucleotide-long duplex DNA, using primers nine and 10 nucleotides long and
E.coli DNA polymerase. Dr. Kleppe described his experiment publicly at the
1969 Gordon Conference on Nucleic Acid [N.S. Templeton.  The Polymerase
Chain Reaction History, Methods, and Applications.  Diagnostic Molecular
Pathology1(1):58-72,1992]. 

	Is there anyone out there has any knowledge on this matter?

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Wasun Chantratita
Department of Clinical Microbiology
Faculty of Associated Medical Sciences
Chiang Mai University
Chiang Mai 50200
Thailand
Tel 053-221122 Ext5086, 5068
Fax 053-221890
Email:asmsi002 at cmu.chiangmai.ac.th
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