Who is the first dicover PCR technique?

Wasun Chantratita asmsi002 at CMU.CHIANGMAI.AC.TH
Tue Mar 8 12:39:39 EST 1994

Dear Netters,

	It may correct to say that Dr. Kary B. Mullis, a research
scientist at the Perkin-Elmer Cetus Corporation in April 1983 was
initially developed a modern PCR technology. However, I recently read an
article described the first PCR experiment was actually performed by Dr.
Kjell Kleppe, a postdoctoral fellow in Dr.  Khorana 's laboratory. This
experiment was a reaction involving two cycles of amplification of a 30
nucleotide-long duplex DNA, using primers nine and 10 nucleotides long and
E.coli DNA polymerase. Dr. Kleppe described his experiment publicly at the
1969 Gordon Conference on Nucleic Acid [N.S. Templeton.  The Polymerase
Chain Reaction History, Methods, and Applications.  Diagnostic Molecular

	Is there anyone out there has any knowledge on this matter?

Wasun Chantratita
Department of Clinical Microbiology
Faculty of Associated Medical Sciences
Chiang Mai University
Chiang Mai 50200
Tel 053-221122 Ext5086, 5068
Fax 053-221890
Email:asmsi002 at cmu.chiangmai.ac.th

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