Sean David Moore
smoore at sas.upenn.edu
Tue Mar 8 12:15:05 EST 1994
I was wondering if anyone knows whether or not Lyticase requires Mg++.
My guess is that it does. I am extracting a surface antigen from an
organism, as well as prepping genomic DNA from the same cells. The
protein extraction requires an overnight incubation at 37C, and I KNOW
that isn't good for my subsequent DNA yield so I would like to help uot as
much as possible perhaps with some EDTA.
Thanks in advance.
SMOORE at SAS.UPENN.EDU
More information about the Methods